Biliary proteins and the nucleation defect in cholesterol cholelithiasis.
Biliary proteins and the nucleation defect in cholesterol cholelithiasis.
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胆汁蛋白和胆固醇胆石症的成核缺陷。
DOI：
10.1016/0016-5085(87)90959-0
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发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
S. Strasberg
中科院分区：
文献类型：
--
作者：
S. Gallinger;P. Robert;C. Harvey;C. Petrunka;R. G. Ilson;S. Strasberg
进行了一项研究，以确定胆囊蛋白的差异是否可能存在于快速成核胆汁患者中。用荧光法测定胆囊和肝胆汁蛋白浓度。该方法通过独立的水解和氨基酸分析技术进行了验证。胆固醇结石患者的胆囊胆汁蛋白浓度明显高于无胆囊疾病患者或色素结石患者。胆固醇结石组蛋白浓度与体外成核时间相关。用色谱法和梯度超离心法纯化胆囊胆汁蛋白。来自胆固醇结石患者的蛋白质加速了对照胆汁的成核时间，而来自对照的蛋白质几乎没有影响。有和没有胆固醇胆结石的人的肝胆汁蛋白浓度相似。测定了各种溶质的胆肝比。胆固醇结石组的蛋白质比例可以直接用胆囊中的水再吸收来解释，而非胆固醇结石患者的蛋白质比例非常低，这表明他们的胆囊通过蛋白质吸收或胆囊吸水过程中的降解等过程减少了蛋白质质量。
A study was performed to determine whether differences in gallbladder proteins might be present in patients with rapidly nucleating bile. Gallbladder and hepatic bile protein concentrations were measured using a fluorometric assay. The method was validated by an independent technique, i.e., hydrolysis and amino acid analysis. Persons with cholesterol gallstones had significantly higher gallbladder bile protein concentrations than patients without gallbladder disease or patients with pigment stones. The protein concentration correlated with the in vitro nucleation time in the cholesterol stone group. Gallbladder bile proteins were also purified by chromatography and gradient ultracentri f ugation. Proteins from patients with cholesterol gallstones accelerated the nucleation time of control bile, whereas protein from controls had little effect. Hepatic bile protein concentrations were similar in persons with and without cholesterol gallstones. The gallbladderto-hepatic bile ratios of a variety of solutes were examined. The ratio for protein in the cholesterol gallstone group can be explained straightforwardly by water reabsorption in the gallbladder, whereas the very low ratio in patients without -cholesterol gallstones suggests that their gallbladders reduce protein mass by a process such as protein absorption or degradation during water absorption in the gallbladder.
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胆汁蛋白质。
DOI：
10.1172/jci111204
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Holzbach,RT;Kibe,A;Thiel,E;Howell,JH;Marsh,M;Hermann,RE
通讯作者：
Hermann,RE
牛磺胆酸盐对混合脂质胶束大小的影响及其与大鼠胆汁中色素和蛋白质的关系。
影响因子：
6.5
作者：
Reuben,A;Howell,KE;Boyer,JL
通讯作者：
Boyer,JL
人类胆汁中高、低和极低密度脂蛋白的载脂蛋白。
影响因子：
6.5
作者：
Sewell,RB;Mao,SJ;Kawamoto,T;LaRusso,NF
通讯作者：
LaRusso,NF
影响因子：
15.9
作者：
LEE, SP;LAMONT, JT;CAREY, MC
通讯作者：
CAREY, MC