Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species:: screening of strains and optimization of omega-3 production
Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species:: screening of strains and optimization of omega-3 production
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DOI：
10.1007/s00253-006-0419-1
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发表时间：
2006-10-01
影响因子：
5
通讯作者：
Barrow, Colin J.
中科院分区：
文献类型：
--
作者：
Burja, Adam M.;Radianingtyas, Helia;Barrow, Colin J.
一项针对来自19个不同大西洋加拿大地点的Thraustochytrids(海洋真菌原生动物)的隔离计划被执行。对68个菌株的生物量、总脂肪酸(TFA)、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)含量进行了筛选。脂肪酸甲酯分析结果根据脂肪酸谱划分出4个明显的类群，生物量在0.1g~2.3g L-1之间，脂肪含量在27.1~321.14 mg g(-1)之间，环境保护剂含量在2.97~21.25mgg(-1)之间，DHA含量在5.18~83.63mgg(-1)之间。ONC-T18，随后被选择用于进一步的操作。经18SRRNA基因测序鉴定，ONC-T18为一株与纹状Thraustochytrium T91-6亲缘关系最近的菌株，在优化的发酵条件下，ONC-T18的生物量可达28.0g L-1，总脂肪酸81.7%，DHA(w/w)31.4%，L DHA 4.6g。此外，该菌株还能产生类胡萝卜素和叶黄素、虾青素、玉米黄质、角黄素、紫锥酮和β-胡萝卜素。考虑到该菌株与商业菌株(如裂殖酵母)相比具有令人印象深刻的生产力。SR21(仅含50%TFA)，再加上其在经济的氮素和非常低的盐浓度(2g L-1)下生长的能力，ONC-T18被视为放大和商业化的理想候选者。
An isolation program targeting Thraustochytrids (marine fungoid protists) from 19 different Atlantic Canadian locations was performed. Sixty-eight isolates were screened for biomass, total fatty acid (TFA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) content. Analysis of fatty acid methyl ester results discerned four distinctive clusters based on fatty acid profiles, with biomass ranging from 0.1 to 2.3 g L-1, and lipid, EPA, and DHA contents ranging from 27.1 to 321.14, 2.97 to 21.25, and 5.18 to 83.63 mg g(-1) biomass, respectively. ONC-T18, was subsequently chosen for further manipulations. Identified using 18S rRNA gene sequencing techniques as a Thraustochytrium sp., most closely related to Thraustochytrium striatum T91-6, ONC-T18 produced up to 28.0 g L-1 biomass, 81.7% TFA, 31.4% (w/w biomass) DHA, and 4.6 g L-1 DHA under optimal fermentation conditions. Furthermore, this strain was found to produce the carotenoids and xanthophylls astaxanthin, zeaxanthin, canthaxanthin, echinenone, and beta-carotene. Given this strain's impressive productivity when compared to commercial strains, such as Schizochytrium sp. SR21 (which has only 50% TFA), coupled with its ability to grow at economical nitrogen and very low salt concentrations (2 g L-1), ONC-T18 is seen as an ideal candidate for both scale-up and commercialization.