Antimicrobial resistance genes are enriched in aerosols near impacted urban surface waters in La Paz, Bolivia
Antimicrobial resistance genes are enriched in aerosols near impacted urban surface waters in La Paz, Bolivia
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DOI：
10.1016/j.envres.2021.110730
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发表时间：
2021-01-14
影响因子：
8.3
通讯作者：
Brown, Joe
中科院分区：
文献类型：
--
作者：
Ginn, Olivia;Nichols, Dennis;Brown, Joe
抗生素耐药性对全球健康构成重大威胁。了解抗生素耐药性在环境介质中的出现和传播对控制策略的设计至关重要。由于抗生素抗性基因（ARGs）可能从污染的点源雾化并在局部环境中更广泛地传播，我们评估了玻利维亚拉巴斯城市气溶胶中的ARGs，那里的废水通过工程地表水通道流经人口稠密的城市核心。我们通过ddPCR定量了关键的ARGs和一个移动整合子（MI），并通过两年多的培养在污水流附近的地点培养大肠杆菌作为粪便指标。ARG靶点代表了主要的抗生素群——四环素类（tetA）、氟喹啉类（qnrB）和β -内酰胺类（bla(TEM)），而MI （intI1）代表了遗传物质移动的潜力。大多数空气样品（82%）的可检测目标高于实验确定的LOD：最常见的是bla（TEM）和intI1（分别为68%和47%），其次是tetA和qnrB（分别为17%和11%）。阳性空气样本中的ARG和MI密度范围为1.3 x 10(1)至6.6 x 10(4)个基因拷贝/m(3)空气。此外，我们在空气中检测到可培养的大肠杆菌（52%的样本）
Antibiotic resistance poses a major global health threat. Understanding emergence and dissemination of antibiotic resistance in environmental media is critical to the design of control strategies. Because antibiotic resistance genes (ARGs) may be aerosolized from contaminated point sources and disseminated more widely in localized environments, we assessed ARGs in aerosols in urban La Paz, Bolivia, where wastewater flows in engineered surface water channels through the densely populated urban core. We quantified key ARGs and a mobile integron (MI) via ddPCR and E. coli spp. as a fecal indicator by culture over two years during both the rainy and dry seasons in sites near wastewater flows. ARG targets represented major antibiotic group-s-tetracyclines (tetA), fluoroquinolines (qnrB), and beta-lactams (bla(TEM))-and an MI (intI1) represented the potential for mobility of genetic material. Most air samples (82%) had detectable targets above the experimentally determined LOD: most commonly bla(TEM) and intI1 (68% and 47% respectively) followed by tetA and qnrB (17% and 11% respectively). ARG and MI densities in positive air samples ranged from 1.3 x 10(1) to 6.6 x 10(4) gene copies/m(3) air. Additionally, we detected culturable E. coli in the air (52% of samples