Observation of sGAG content of human hip joint cartilage in different old age groups based on EPIC micro-CT.
Observation of sGAG content of human hip joint cartilage in different old age groups based on EPIC micro-CT.
复制标题
DOI：
10.3109/03008207.2015.1009052
复制
发表时间：
2015-04
影响因子：
2.9
通讯作者：
Qin YX
中科院分区：
文献类型：
--
作者：
Li XF;Cai XR;Fan F;Niu HJ;Li SY;Li DY;Fan YB;Qin YX
目的：应用离子型造影剂平衡分配（EPIC）显微CT技术，观察老年人髋关节软骨中硫酸化糖胺聚糖（sGAG）含量的增龄性变化。从髋部骨折患者（年龄51-96岁）中收集了70个人髋部软骨-骨样本，并将其分为五组（每个年龄组10岁）。首先将它们浸入20%浓度的造影剂泛影葡胺（MD）中，在37 °C下持续6 h，然后通过micro-CT扫描。扫描后，用甲苯胺蓝对样品进行sGAG染色。通过图像处理计算X射线衰减和sGAG光密度。然后分析X射线衰减和sGAG光密度之间的相关性。50-80岁组软骨X线平均衰减值比80岁组增加18.81%（P<0.01），90岁组比80岁组减少7.15%（P <0.01）。50-80岁组浅层和中层平均衰减分别增加31.60%和44.68%（P<0.01），90岁组分别减少4.67%和6.05%。而深层则无明显变化。sGAG光密度与X射线衰减呈线性相关（r =-0.91，p<0.01）。老年人髋关节软骨sGAG含量随增龄而变化。浅层和中层的变化较深层明显。
To observe the age-related changes of sulfated glycosaminoglycan (sGAG) content of hip joint cartilage of elderly people based on Equilibrium Partitioning of an Ionic Contrast Agent (EPIC) micro-CT. Seventy human hip cartilage–bone samples were collected from hip-fracture patients (ages 51–96) and divided into five groups (10 years in an age group). They were first immersed in 20% concentration of the contrast agent Meglumine Diatrizoate (MD) for 6 h at 37 °C, and then scanned by micro-CT. Following scanning, samples were stained for sGAG with toluidine blue. The X-ray attenuation and sGAG optical density were calculated by image processing. The correlation between X-ray attenuation and sGAG optical density was then analyzed. The X-ray mean attenuation of the cartilage increased by 18.81% from the 50–80 age groups (p<0.01), but decreased by 7.15% in the 90 age group compared to the 80 age group. The X-ray mean attenuation of the superficial layer and middle layer increased by 31.60 % and 44.68% from the 50–80 age groups, respectively (p<0.01), but reduced by 4.67% and 6.05% separately in the 90 age group. However, the deep layer showed no significant change with aging. The sGAG optical density showed a linear correlation (r = −0.91, p<0.01) with the X-ray attenuation. The sGAG content of hip joint cartilage varied with aging in elderly people. The changes in superficial layer and middle layer were more evident than deep layer.