Focal modulation microscopy with annular apertures: A numerical study
Focal modulation microscopy with annular apertures: A numerical study
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DOI：
10.1002/jbio.200900110
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发表时间：
2010-07
影响因子：
2.8
通讯作者：
W. Gong;Ke Si;Nanguang Chen;C. Sheppard
中科院分区：
文献类型：
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作者：
W. Gong;Ke Si;Nanguang Chen;C. Sheppard
介绍了环形孔径焦调制显微镜（AFMM）的成像方法。讨论了空间分辨率。与共聚焦显微镜相比，AFMM可以同时提高轴向和横向分辨率。通过调整环形物镜孔径的宽度，AFMM可以从最佳空间分辨率性能调整到最高信号水平。本文还对背景抑制能力进行了研究，表明AFMM具有进一步提高成像穿透深度的潜力。最后，对AFMM的优化结构进行了阐述。（©2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim）
Image formation of focal modulation microscopy with annular aperture (AFMM) is presented. The spatial resolution is discussed. Compared with confocal microscopy, AFMM can simultaneously enhance the axial and transverse resolution. By adjusting the width of the annular objective aperture, AFMM can be adjusted from best spatial resolution performance to highest signal level. The capability of background rejection is also investigated, showing AFMM has the potential to further increase the imaging penetration depth. Finally, an optimal configuration of AFMM is elaborated. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)