A detailed analysis of open-field habituation and behavioral and neurochemical antidepressant-like effects in postweaning enriched rats
A detailed analysis of open-field habituation and behavioral and neurochemical antidepressant-like effects in postweaning enriched rats
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DOI：
10.1016/j.bbr.2008.08.014
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发表时间：
2009-01-30
影响因子：
2.7
通讯作者：
Fornaguera, Jaime
中科院分区：
文献类型：
--
作者：
Brenes, Juan C.;Padilla, Michael;Fornaguera, Jaime
我们以前的工作表明，雄性Sprague-Dawley大鼠饲养在社会隔离，标准住房和环境丰富的不同，在他们的自发开放领域的活动，并在一些神经行为抑郁样参数。在这里，我们通过使用较短的断奶后饲养期（1个月）并包括额外的评估来扩展这一证据。首先，为了更好地表征饲养条件下的探索策略，我们详细分析了第一分钟和10分钟期间的自发活动。第二，我们询问是否开放领域的活动的变化与高架十字迷宫的基础焦虑水平。第三，分析强迫游泳试验中的行为，然后评估海马去甲肾上腺素和5-羟色胺的组织水平。通过相关分析，探讨神经递质与强迫游泳行为的可能关系。我们发现，在不改变十字迷宫行为的情况下，饲养条件（i）在第一分钟和10分钟的运动习惯化和感觉运动探索上存在差异：（ii）对梳理时间、其顺序成分以及梳理与运动参数之间的关系存在差异;（iii）修改强迫游泳行为和海马去甲肾上腺素，5-羟色胺的浓度，其营业额;和（iv）神经递质和强迫游泳行为之间产生不同的相关模式。总的来说，丰富的环境加速了开放领域的习惯化，并导致行为和神经化学抗抑郁剂样的影响。与此相反，隔离饲养强烈损害习惯化和简单的信息处理，但表现出边缘影响抑郁样行为和海马神经化学。目前的研究结果表明，差异养育不仅是一个有用的程序来研究行为的可塑性或刚性的早期经验，但也建模一些发展的保护性或潜在的抑郁症的风险因素。(C)2008 Elsevier B. V.保留所有权利。
Our previous work has shown that male Sprague-Dawley rats reared in social isolation, standard housing and environmental enrichment differ in their spontaneous open-field activity and in some neurobehavioral depressive-like parameters. Here, we extended this evidence by using a shorter postweaning rearing period (I month) and including additional evaluations. First, in order to obtain a better characterization of the exploratory strategies among rearing conditions we analyzed in detail the spontaneous activity at the first minute and during the 10-min session. Second, we asked whether the changes in open-field activity were related with basal anxiety levels in the elevated plus-maze. Third, behavior in the forced-swimming test was analyzed and afterward, the tissue levels of hippocampal norepinephrine and serotonin were assessed. The possible relationship between neurotransmitters and forced-swimming behavior were explored through correlation analyses. We found that rearing conditions (i) differed on locomotor habituation and on sensory-motor exploration at the first minute and during the 10-min session without modifying the plus-maze behavior: (ii) affected differentially the grooming time, its sequential components, and the relationship between grooming and locomotor parameters; (iii) modified forced-swimming behavior and the hippocampal concentration of norepinephrine, serotonin, and its turnover; and (iv) produced different correlation patterns between both neurotransmitters and forced-swimming behaviors. Overall, environmental enrichment accelerated open-field habituation and led to behavioral and neurochemical antidepressant-like effects. In contract, isolation rearing strongly impaired habituation and simple information processing, but showed marginal effects on depressive-like behavior and on hippocampal neurochemistry. The current results suggest that differential rearing is not only a useful procedure to study behavioral plasticity or rigidity in response to early experience, but also to modeling some developmental protective or risk factors underlying depressive disorders. (C) 2008 Elsevier B.V. All rights reserved.