Simple method for rapid measurement of trichloroethylene and its major metabolites in biological samples
Simple method for rapid measurement of trichloroethylene and its major metabolites in biological samples
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DOI：
10.1016/s0378-4347(99)00282-0
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发表时间：
1999-09-10
期刊：
影响因子：
--
通讯作者：
Bruckner, JV
中科院分区：
文献类型：
--
作者：
Muralidhara, S;Bruckner, JV
建立了一种简单、快速、灵敏的同时测定三氯乙烯（TCE）及其主要代谢产物（即，三氯乙酸、三氯乙醇和二氯乙酸）。该方法包括向密封小瓶中的血液或组织匀浆中加入溶解剂（水、硫酸、甲醇; 6：5：1; v/v/v），随后进行气相色谱顶空分析。该程序应该是有用的医疗监测TCE暴露以及在实验工作中，特别是药代动力学和药效学研究有关的TCE致癌作用。(C)1999 Elsevier Science B. V.保留所有权利。
A simple and rapid, yet sensitive technique was developed for concurrent measurement of trichloroethylene (TCE) and its major metabolites (i.e., trichloroacetic acid, trichloroethanol and dichloroacetic acid) in blood and in solid tissues. The method involves addition of an esterizer (water, sulfuric acid, methanol; 6:5:1; v/v/v) to blood or tissue homogenate in sealed vials, and subsequent gas chromatographic headspace analysis. The procedure should be useful in medical monitoring of TCE exposure as well as in experimental work, notably pharmacokinetic and pharmacodynamic studies pertaining to TCE carcinogenesis. (C) 1999 Elsevier Science B.V. All rights reserved.