Molecular epidemiology analysis of Brucella in tricks in Xinjiang Uygur autonomous region.
Molecular epidemiology analysis of Brucella in tricks in Xinjiang Uygur autonomous region.
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DOI：
10.3760/cma.j.issn.2095-4255.2019.12.001
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发表时间：
2019-01-01
影响因子：
--
通讯作者：
Sheng, J. L.
中科院分区：
文献类型：
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作者：
Jiang, Meng-meng;Yang, Mei-hua;Sheng, J. L.
Objective: To carry out a investigation on molecular epidemiological features of tick-borne BruceIla in Xinjiang Uygur Autonomous Region (Xinjiang), and to provide a scientific basis for formulation of effective preventive and control measures. Methods: In 2016 - 2018, parasitic ticks (including engorged females) were collected on the body surface of livestock in 10 counties (cities) along the border of Xinjiang. The free-living ticks were collected by flagging method in Alashankou. The engorged female was placed in a breathable insect tube for spawning, each egg batch was divided into two parts: one part was tick eggs, while the second part was allowed further larval development. All ticks were identified by molecular biology (16S rRNA) identification. Tick DNA was extracted, PCR was performed based on Brucella omp22 and 15711, and amplification products were sequenced and analyzed by BLAST. Results A total of 1 084 ticks were collected in 11 counties (cities), of them 747 were parasitic ticks (including 34 engorged females) and 337 were free-living ticks. Based on 16S rRNA identification, 1084 ticks belonged to 4 genera and 5 species, and the proportions of Dermacentor nuttalli. Dermarentor marginatus, Haemaphysalis punctata, Hyalomma asiaticum and Rhipicepludas turanicus were 29.43% (319/1 084), 16.51% (179/1 084), 10.42%(113/1 084), 37.27% (404/1 084), and 6.37% (69/1 084), respectively. A total of 214 Bruce!lapositive nucleic acid samples were detected, the positive rate was 19.74%. The parasitic ticks' positive rate was 25.30% (189/747). and the freeliving ticks' positive rate was 7.42% (25/337), parasitic ticks' positive rate was higher than that of freeliving ticks (x2 = 46.873, P < 0.05). Two Brucella. melitensis nucleic acid samples were detected in 34 engorged femalestick eggs developmental stage. and one Brucetla melitensis nucleic acid sample was detected in 22 tick eggslarvae developmental stage. Conclusions: Brucella is widely distributed in parasitic ticks and freeliving ticks in Xinjiang border areas, and the parasitic ticks' positive rate is obviously higher than that of freeliving ticks. The Bruceila melitensis has potential transovarian transmission and transstadial transmission in ticks. In the prevention and control of livestock brucellosis. the work of killing ticks should be strengthened. including parasitic ticks on the body surface and freeliving ticks in the environment.
