Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine estrous cycle
Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine estrous cycle
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DOI：
10.1016/s0304-4165(97)00136-0
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发表时间：
1998-03-12
影响因子：
3
通讯作者：
Hanukoglu, I
中科院分区：
文献类型：
--
作者：
Rapoport, R;Sklan, D;Hanukoglu, I
类固醇激素生物合成的反应：伴随着氧自由基的形成。我们测定了牛黄体不同发育阶段的抗氧化剂和抗氧化酶的水平，以研究它们与类固醇生成状态的相关性。发情周期同步奶牛的血浆孕酮浓度升高，直到16天，然后迅速下降，在黄体退化。类固醇生成细胞色素P450 SCC和肾上腺素还蛋白的水平与血浆孕酮的变化一致。在抗氧化酶检查，超氧化物歧化酶和过氧化氢酶的活性显示模式最相似的血浆孕酮。过氧化氢酶和超氧化物歧化酶活性从发情周期的第6天到第16天增加6-8倍，然后在黄体抑制期间下降。抗坏血酸和β-胡萝卜素与P450 SCC和血浆孕酮水平呈低但显着的相关性。两种亲脂性抗氧化剂在黄体中的分布有很大差异。从第6天到第16天，β-胡萝卜素浓度增加约6倍，并且在退化组织中降低。α-生育酚在第6天和第9天之间显示出3倍的增加，随后迅速降低。因此，在黄体中期类固醇生成高峰时，α-生育酚水平降低，但β-胡萝卜素水平升高。一些抗氧化酶和化合物的水平与孕酮水平之间的相关性表明，抗氧化机制被激活，以科普类固醇激素依赖的氧自由基的形成在牛黄体。(C)1998年Elsevier Science B.V.
The reactions of steroid hormone biosynthesis: are accompanied by formation of oxygen radicals. We determined the levels of some antioxidants and antioxidative enzymes at different developmental stages of bovine corpora lutea to examine their correlation with steroidogenic status. Plasma progesterone concentrations of estrous cycle synchronized cows increased until day 16, and then decreased rapidly during luteal regression. The levels of steroidogenic cytochrome P450scc and adrenodoxin paralleled the changes in plasma progesterone. Among the antioxidative enzymes examined, the SOD and catalase activities showed patterns most similar to plasma progesterone. Catalase and SOD activities increased 6-8 fold from day 6 to 16 of the estrous cycle and then decreased during the luteal repression. Ascorbate and beta-carotene showed low but significant correlation with P450scc and plasma progesterone levels. The profiles of two lipophilic antioxidants in corpora lutea were very different. beta-carotene concentration increased by approximately 6 fold from day 6 to 16, and decreased in regressive tissue. alpha-tocopherol showed a 3 fold increase between days 6 and 9 followed by a rapid decrease. Thus, at the peak of steroidogenesis at mid-luteal phase alpha-tocopherol levels decreased, but beta-carotene levels increased. The correlation between the levels of some antioxidant enzymes and compounds with progesterone levels indicates that antioxidative mechanisms are activated to cope with steroidogenesis dependent oxyradical formation in the bovine corpus luteum. (C) 1998 Elsevier Science B.V.