Determination of nicotine, cotinine and caffeine in meconium using high-performance liquid chromatography
Determination of nicotine, cotinine and caffeine in meconium using high-performance liquid chromatography
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DOI：
10.1016/s0378-4347(97)00619-1
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发表时间：
1998-04-10
期刊：
影响因子：
--
通讯作者：
Baranowska, I
中科院分区：
文献类型：
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作者：
Baranowski, J;Pochopien, G;Baranowska, I
建立了一种高效液相色谱-二极管阵列检测法测定胎粪中尼古丁及其代谢产物可替宁和咖啡因的方法。该方法适用于评估胎儿暴露于烟草烟雾。分析物在色谱前通过固相萃取进行提取。在30份胎粪样本中，11份对可替宁（20-86 ng/g）呈阳性，27份对咖啡因（10-45 ng/g）呈阳性。样本中不存在尼古丁，因为其快速代谢为可替宁。(C)1998年Elsevier Science B.V.
A high-performance liquid chromatographic method with diode-array detection for the determination of nicotine and its metabolites, cotinine and caffeine, in meconium is described. This method is suitable to assess foetus exposure to tobacco smoke. The analytes were extracted by solid-phase extraction before chromatography. From among 30 meconium samples 11 were positive for cotinine (20-86 ng/g) and 27 for caffeine (10-45 ng/g). No nicotine was present in the samples because of its rapid metabolism into cotinine. (C) 1998 Elsevier Science B.V.