The genome analysis of CandidatusBurkholderia crenata reveals that secondary metabolism may be a key function of the Ardisia crenata leaf nodule symbiosis
The genome analysis of CandidatusBurkholderia crenata reveals that secondary metabolism may be a key function of the Ardisia crenata leaf nodule symbiosis
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DOI：
10.1111/1462-2920.13184
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发表时间：
2016-08-01
影响因子：
5.1
通讯作者：
Eberl, Leo
中科院分区：
文献类型：
--
作者：
Carlier, Aurelien;Fehr, Linda;Eberl, Leo
大多数紫金牛属物种在特化的叶结节内窝藏伯克霍尔德氏菌属细菌。这种细菌是遗传性传播的，尚未在宿主外培养。由于植物在没有共生体的情况下不能超过幼苗阶段，因此共生被认为是强制性的。首次测定了白僵菌Burkholderia crenata（Ca. B. crenata）的根瘤共生体。Ca.B. crenata是迄今为止最小的伯克霍尔德氏菌基因组。它包含大量的插入序列和假基因，并显示与还原基因组进化一致的特征。基因组不编码通常与植物共生有关的功能，如固氮和植物激素代谢。然而，我们确定了独特的基因预测的作用，在次生代谢的基因组中的钙。B。钝齿。具体而言，我们提供的证据表明，细菌共生体负责合成的化合物FR 900359，一个环状缩酚酸肽与生物医学特性以前分离的A. crenata叶。
A majority of Ardisia species harbour Burkholderia sp. bacteria within specialized leaf nodules. The bacteria are transmitted hereditarily and have not yet been cultured outside of their host. Because the plants cannot develop beyond the seedling stage without their symbionts, the symbiosis is considered obligatory. We sequenced for the first time the genome of CandidatusBurkholderia crenata (Ca.B. crenata), the leaf nodule symbiont of Ardisia crenata. The genome of Ca.B. crenata is the smallest Burkholderia genome to date. It contains a large amount of insertion sequences and pseudogenes and displays features consistent with reductive genome evolution. The genome does not encode functions commonly associated with plant symbioses such as nitrogen fixation and plant hormone metabolism. However, we identified unique genes with a predicted role in secondary metabolism in the genome of Ca. B. crenata. Specifically, we provide evidence that the bacterial symbionts are responsible for the synthesis of compound FR900359, a cyclic depsipeptide with biomedical properties previously isolated from leaves of A.crenata.