Increased density but not prevalence of gametocytes following drug treatment of Plasmodium falciparum
Increased density but not prevalence of gametocytes following drug treatment of Plasmodium falciparum
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DOI：
10.1016/j.trstmh.2005.04.021
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发表时间：
2006-02-01
影响因子：
2.2
通讯作者：
Babiker, HA
中科院分区：
文献类型：
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作者：
Ali, E;Mackinnon, MJ;Babiker, HA
在苏丹东部的一个村庄，我们监测了接受氯喹（CQ）和磺胺多辛-乙胺嘧啶（SP; Fansidar(R)）治疗的患者治疗后的恶性疟原虫。分别于传播季节第0天（处理前）、第7天、第14天和第21天（处理后）检测寄生虫。2个月后（第80天）在旱季开始时再次取样。显微镜下检测无性形态和配子细胞，逆转录聚合酶链反应（RT-PCR）检测配子细胞特异性蛋白pfs25和pfg377的表达。显微镜观察显示，CQ和SP处理后配子体携带量显著增加，在第7天至第14天达到最大值。然而，当RT-PCR测量时，配子细胞率在第0天与第7天或第14天之间没有显著差异。治疗后第80天，RT-PCR配子细胞率急剧下降，但此时CQ和sp处理组仍为33%和8%。恶性疟原虫对氯喹（氯喹耐药转运体pfcrt和多药耐药pfmdr1）和乙胺嘧啶（二氢叶酸还原酶dhfr）耐药相关的等位基因在治疗开始时出现频率较高，在两种药物治疗后随着时间的推移进一步增加。耐药寄生虫感染往往比药物敏感感染有更高的配子体流行率。(C) 2005年皇家热带医学与卫生学会。Elsevier Ltd.出版。版权所有。
We monitored post-treatment Plasmodium falciparum among patients treated with chloroquine (CQ) and sutfadoxine-pyrimethamine (SP; Fansidar(R)) in a village in eastern Sudan. Parasites were examined on day 0 (pre-treatment), day 7, day 14 and day 21 (post-treatment) during the transmission season. A further sample was taken 2 months later (day 80) at the start of the dry season. Asexual forms and gametocytes were detected by microscopy, and reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect expression of gametocyte-specific proteins pfs25 and pfg377. Gametocyte carriage, as revealed by microscopy, increased significantly following CQ and SP treatment, reaching a maximum between days 7 and 14. When measured by RT-PCR, however, there was no significant difference in gametocyte rate between day 0 and days 7 or 14. RT-PCR gametocyte rates dropped dramatically by day 80 post treatment but were still 33% and 8% in the CQ- and SP-treated group at this time. Alleles associated with drug resistance of P falciparum to chloroquine (the chloroquine resistance transporter, pfcrt, and multidrug resistance, pfmdr1) and to pyrimethamine (dihydrofolate reductase, dhfr) were seen at a high frequency at the beginning of treatment and increased further through time following both drug treatments. Infections with drug-resistant parasites tended to have higher gametocyte prevalence than drug-sensitive infections. (C) 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.