CHALCONE SYNTHASE-LIKE GENES ACTIVE DURING COROLLA DEVELOPMENT ARE DIFFERENTIALLY EXPRESSED AND ENCODE ENZYMES WITH DIFFERENT CATALYTIC PROPERTIES IN GERBERA-HYBRIDA (ASTERACEAE)
CHALCONE SYNTHASE-LIKE GENES ACTIVE DURING COROLLA DEVELOPMENT ARE DIFFERENTIALLY EXPRESSED AND ENCODE ENZYMES WITH DIFFERENT CATALYTIC PROPERTIES IN GERBERA-HYBRIDA (ASTERACEAE)
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DOI：
10.1007/bf00042037
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发表时间：
1995-04-01
影响因子：
5.1
通讯作者：
TEERI, TH
中科院分区：
文献类型：
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作者：
HELARIUTTA, Y;ELOMAA, P;TEERI, TH
最近对查尔酮合酶(CHS)和相关的二苯乙烯合酶(STS)的研究表明，植物中CHS类基因的结构已经进化成不同的形式，其成员对不同但相关的酶活性具有不同的调控和编码能力。我们通过分析非洲菊花冠发育过程中活跃的三个基因的结构、表达模式和相应酶的催化特性，研究了类chs基因的多样性。这些表达模式表明，chs类基因代表了在非洲菊器官分化过程中活跃的三个不同的遗传程序。Gchs1和gchs3编码典型的CHS酶，它们的基因表达模式与花冠发育过程中黄酮醇(gchs1，gchs3)和花青素(Gchs1)的合成存在时间相关性。Gchs2不同。表达模式与色素模式无关，氨基酸序列与典型CHSS的一致性有较大偏离，催化性质也不同。这些数据表明，它代表了chs及其相关基因大超家族中的一个新成员。
Recent studies on chalcone synthase (CHS) and the related stilbene synthase (STS) suggest that the structure of chs-like genes in plants has evolved into different forms, whose members have both different regulation and capacity to code for different but related enzymatic activities. We have studied the diversity of chs-like genes by analysing the structure, expression patterns and catalytic properties of the corresponding enzymes of three genes that are active during corolla development in Gerbera hybrida. The expression patterns demonstrate that chs-like genes are representatives of three distinct genetic programmes that are active during organ differentiation in gerbera. Gchs1 and gchs3 code for typical CHS enzymes, and their gene expression pattern temporally correlates with flavonol (gchs1, gchs3) and anthocyanin (gchs1) synthesis during corolla development. Gchs2 is different. The expression pattern does not correlate with the pigmentation pattern, the amino acid sequence deviates considerably from the consensus of typical CHSs, and the catalytic properties are different. The data indicate that it represents a new member in the large superfamily of chs and chs-related genes.