Identification and analysis of a collagenolytic activity in Streptococcus mutans
Identification and analysis of a collagenolytic activity in Streptococcus mutans
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DOI：
10.1007/s002849900143
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发表时间：
1997-01-01
影响因子：
2.6
通讯作者：
Dao, ML
中科院分区：
文献类型：
--
作者：
Jackson, RJ;Lim, DV;Dao, ML
变形链球菌（Streptococcus mutans）是一种重要的冠龋致病菌，其与各种来源的胶原蛋白结合的能力与牙根龋有关。变形链球菌能结合并破坏羊膜的胶原纤维。与在不存在蔗糖的情况下生长的细胞相比，在蔗糖生长的细胞中观察到较低水平的AGPA水解活性。抑制研究表明，胶原酶活性的模式特征，抑制1，10-菲咯啉和EDTA，但不是由苯甲基磺酰氟（PMSF）。此外，从S.变形杆菌和溶组织梭菌胶原酶的抗血清。明胶酶谱分析表明，S.变异菌可能是牙根腐烂的一个重要毒力因子。
Streptococcus mutans is an important pathogen in coronal caries and is implicated in dental root decay by its ability to bind collagen from various sources, In the present study, electron microscopic analysis demonstrated the ability of S. mutans to bind and to disrupt collagen fibrils of the amniotic membrane, The synthetic peptide FALGPA, which is similar in structure to collagen, was degraded by S. mutans, with a lower level of FALGPA hydrolytic activity observed in sucrose-grown cells compared with cells grown in the absence of sucrose. Inhibition studies of FALGPA hydrolytic activity showed a pattern characteristic of collagenase activity, with inhibition by 1,10-phenanthroline and EDTA, but not by phenylmethylsulfonyl fluoride (PMSF). Additionally, immunological cross-reactivity was observed between proteins from disrupted cells of S. mutans and antiserum to collagenase from Clostridium histolyticum. Gelatinolytic activity was demonstrated by gelatin zymogram analysis, These findings suggest that collagenolytic activity by S. mutans may be an important virulence factor in dental root decay.