Inhibition of PhIP-induced mammary carcinogenesis in female rats by ingestion of freeze-dried beer
Inhibition of PhIP-induced mammary carcinogenesis in female rats by ingestion of freeze-dried beer
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DOI：
10.1016/j.canlet.2005.04.020
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发表时间：
2006-04-08
期刊：
影响因子：
9.7
通讯作者：
Kondo, K
中科院分区：
文献类型：
--
作者：
Nozawa, H;Nakao, W;Kondo, K
本研究旨在探讨啤酒中的非酒精成分对2-氨基-1-甲基-6-苯基咪唑并[4，5-B]吡啶（PhIP）诱发乳腺癌的调节作用。将6周龄的雌性Sprague-Dawley（SD）大鼠分为4组（n = 26-30），并喂食高脂肪饮食或含有1、2或4%冻干啤酒（FD啤酒）的高脂肪饮食。开始喂食后一周，大鼠通过管饲法接受PhIP，剂量为85 mg/kg，每周四次，持续2周。对照组和实验组大鼠的体重或饮食摄入量无差异。每周观察可触及的肿瘤表明，在整个实验中，2%和4% FD啤酒组的肿瘤发病率和肿瘤多样性低于对照组。在22周实验结束时对肿瘤病变进行病理学检查。FD啤酒摄入量以剂量依赖性方式抑制肿瘤发展。与对照组（73.3%和1.8 +/-0.7）相比，喂食含4% FD饮食的组的肿瘤发生率（38.5%）和肿瘤多样性（0.8 +/-0.4）显著降低。补充FD啤酒3周与PhIP治疗一起导致肝脏GST活性增加，肝脏CYP 1A 2活性降低和乳腺组织中DNA加合物数量减少，尽管这些值并不显著。总之，我们的研究结果表明，摄入FD啤酒可以降低杂环胺致癌的风险。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
This study evaluated the modulating effect of non-alcoholic constituents of beer on 2-amino-l-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced mammary carcinogenesis. Female Sprague-Dawley (SD) rats at 6 weeks of age were divided into four groups (n = 26-30) and fed either a high fat diet or high fat diets containing 1, 2 or 4% freeze-dried beer (FD beer). One week after the start of feeding, rats received PhIP at a dose of 85 mg/kg by gavage four times weekly for 2 weeks. There were no differences in the body weights or diet intakes of rats between the control and the experimental groups. Weekly observation of palpable tumors indicated that tumor incidence and tumor multiplicity in the 2 and 4% FD beer groups were lower than in the control group throughout the experiment. Neoplastic lesions were pathologically examined at the end of the 22-weeks experiment. Tumor development was inhibited by FD beer intake in a dose-dependent manner. Tumor incidence (38.5%) and tumor multiplicity (0.8 +/- 0.4) for the group fed with a diet containing 4% FD were significantly reduced as compared with the control group (73.3% and 1.8 +/- 0.7). Supplementation with FD beer for 3 weeks together with the PhIP treatments resulted in increased liver GST activity, decreased liver CYP1A2 activity and a decrease in the number of DNA adducts in the mammary tissue, though these values were not significant. In conclusion, our results suggest that intake of FD beer may reduce the risk of carcinogenesis caused by heterocyclic amines. (c) 2005 Elsevier Ireland Ltd. All rights reserved.