Embryonic origin of the Drosophila brain neuropile.
Embryonic origin of the Drosophila brain neuropile.
复制标题
果蝇脑神经丛的胚胎起源。
DOI：
10.1002/cne.20884
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Hartenstein,Volker
中科院分区：
文献类型：
--
作者：
Younossi-Hartenstein,Amelia;Nguyen,Bidong;Shy,Diana;Hartenstein,Volker
果蝇幼虫脑的神经元是由一组定型的神经母细胞形成的。随着分化的开始，神经母细胞谱系产生轴突束，这些轴突束最初在脑原基的中心形成无分支纤维的支架。随后，轴突精心交织的轴突和树突的乔木，其中，连同鞘样的过程形成的胶质细胞，建立幼虫脑的神经细胞室。通过使用标记物，可视化分化的轴突和神经胶质细胞，我们分析了形成的神经桩隔间和它们的关系，成神经细胞谱系。每个谱系的神经元将其轴突延伸为内聚束（“初级轴突束”）。我们生成了初级轴突束的地图，其可视化了轴突束起源的大脑皮层中初级谱系的位置、轴突束进入神经堆的轨迹以及这些束与神经堆先驱束的早期形成的支架的关系（Nassif et al. [1998] J. Comp. Neurol. 402：10-31）。该图进一步显示了先驱束周围特定位置的神经堆隔室的生长。神经胶质细胞发育的时间过程表明，神经胶质细胞的过程，形成突出的隔膜周围的车厢在幼虫的大脑，出现在胚胎neuropile很晚，明确后车厢本身已经结晶。这表明，空间信息驻留在神经元，而不是神经胶质细胞，指定的位置和初始形状的神经细胞隔室。J. Comp.神经元© 2006 Wiley-Liss，Inc.
Neurons of the Drosophila larval brain are formed by a stereotyped set of neuroblasts. As differentiation sets in, neuroblast lineages produce axon bundles that initially form a scaffold of unbranched fibers in the center of the brain primordium. Subsequently, axons elaborate interlaced axonal and dendritic arbors, which, together with sheath-like processes formed by glial cells, establish the neuropile compartments of the larval brain. By using markers that visualize differentiating axons and glial cells, we have analyzed the formation of neuropile compartments and their relationship to neuroblast lineages. Neurons of each lineage extend their axons as a cohesive tract (“primary axon bundle”). We generated a map of the primary axon bundles that visualizes the location of the primary lineages in the brain cortex where the axon bundles originate, the trajectory of the axon bundles into the neuropile, and the relationship of these bundles to the early-formed scaffold of neuropile pioneer tracts (Nassif et al.[1998] J. Comp. Neurol. 402: 10–31). The map further shows the growth of neuropile compartments at specific locations around the pioneer tracts. Following the time course of glial development reveals that glial processes, which form prominent septa around compartments in the larval brain, appear very late in the embryonic neuropile, clearly after the compartments themselves have crystallized. This suggests that spatial information residing within neurons, rather than glial cells, specifies the location and initial shape of neuropile compartments. J. Comp. Neurol. 497: 981–998, 2006.© 2006 Wiley-Liss, Inc.