Intracellular location of inapparently infecting Chlamydia in synovial tissue from patients with Reiter's syndrome.
Intracellular location of inapparently infecting Chlamydia in synovial tissue from patients with Reiter's syndrome.
复制标题
赖特综合征患者滑膜组织中隐性感染衣原体的细胞内定位。
DOI：
10.1007/bf02918277
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发表时间：
1994
影响因子：
4.4
通讯作者：
Hudson,AP
中科院分区：
文献类型：
--
作者：
Beutler,AM;Whittum-Hudson,JA;Nanagara,R;Schumacher,HR;Hudson,AP
从Reiter综合征（RS）患者的滑膜组织/液中培养沙眼衣原体经常产生阴性结果。然而，我们已经确定了衣原体，RNA在该网站在这样的病人表明，可行的有机体可能存在。在这里，我们通过原位杂交确定滑膜内衣原体的细胞定位。使用衣原体核糖体RNA定向探针，我们表明，从培养阴性RS患者的滑膜组织提供了强杂交，这往往是本地化的滑膜下细胞层，而不是滑膜衬里，在某些情况下，杂交细胞分散通过滑膜。所有的杂交信号都位于宿主细胞内，表明感染性细胞外原体是罕见的或不存在的。这些数据证实了广泛的细胞内存在的不明显的衣原体在滑膜RS患者，并提供了一些洞察滑膜组织的生物体通常的文化阴性。
Culture ofChlamydia trachomatisfrom synovial tissues/fluids from Reiter's syndrome (RS) patients frequently yields negative results. However, we have identified chlamydial, RNA at that site in such patients suggesting that viable organisms may be present. Here we define the cellular location of chlamydia within the synovium via in situ hybridization. Using a chlamydial ribosomal RNA-directed probe, we show that synovial tissue from culture-negative RS patients gives strong hybridization which is often localized to a subsynovial cell layer, rather than to the synovial lining; in some cases, hybridizing cells are dispersed through the synovium. All hybridization signal is located within host cells, indicating that infectious extracellular elementary bodies are rare or absent. These data confirm the extensive intracellular presence of inapparent chlamydia in the synovia of RS patients and provide some insight into the usual culture negativity of synovial tissues for the organism.