Unequal crossingover between homologous chromosomes is not the major mechanism involved in the generation of new alleles at VNTR loci.
Unequal crossingover between homologous chromosomes is not the major mechanism involved in the generation of new alleles at VNTR loci.
复制标题
同源染色体之间的不平等交换并不是 VNTR 基因座产生新等位基因的主要机制。
DOI：
10.1016/0888-7543(89)90076-1
复制
发表时间：
1989
期刊：
影响因子：
4.4
通讯作者：
White,R
中科院分区：
文献类型：
--
作者：
Wolff,RK;Plaetke,R;Jeffreys,AJ;White,R
为了研究当在VNTR位点产生新的等位基因时涉及同源染色体之间的不平等交换的假设，我们使用遗传连锁图来识别VNTR标记位点周围的侧翼标记。选择小卫星探针γ MS 1，因为它检测的高变基因座在种系中以约5%的比率自发产生新等位基因。多点连锁分析将γMS1定位在染色体1p上的一组多态性标记位点内。使用两个最近的侧翼标记CMM8和YNZ2，我们能够根据侧翼标记之间的交叉来表征12个新的等位基因事件。对这些数据的统计分析使我们能够拒绝假设产生新等位基因的事件总是涉及减数分裂时同源染色体之间的不平等交换的模型。
To investigate the hypothesis that unequal exchange between homologous chromosomes is involved when new alleles are generated at VNTR loci, we used genetic linkage maps to identify flanking markers surrounding a VNTR marker locus. The minisatellite probe γMS1 was selected, as the hypervariable locus it detects undergoes spontaneous generation of new alleles in the germline at a rate of approximately 5%. Multipoint linkage analysis placed γMS1 within a cluster of polymorphic marker loci on chromosome 1p. Using the two closet flanking markers, CMM8 and YNZ2, we were able to characterize 12 new-allele events in terms of crossingover between the flanking markers. Statistical analysis of these data has allowed us to reject the model that assumes that events generating new alleles always involve unequal exchange between homologous chromosomes at meiosis.