New quinolizidine alkaloid and insecticidal activity of Sophora secundiflora and Sophora tomentosa against Culex pipiens (Diptera: Culicidae)
New quinolizidine alkaloid and insecticidal activity of Sophora secundiflora and Sophora tomentosa against Culex pipiens (Diptera: Culicidae)
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DOI：
10.1080/14786419.2021.1919108
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发表时间：
2021-04-18
影响因子：
2.2
通讯作者：
Singab, Abdel Nasser B.
中科院分区：
文献类型：
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作者：
Aly, Shaza H.;Elissawy, Ahmed M.;Singab, Abdel Nasser B.
通过对苦参生物碱部位的植物化学研究，分离得到1个新的喹诺利定生物碱(1)13-甲氧基苦参碱和6个已知化合物(2-7)。用不同浓度测定了毛细管库蚊叶片70%甲醇提取物和生物碱对淡色库蚊3龄幼虫的杀虫活性，并记录其死亡率。苦参提取物24 h的半数致死浓度为3.11 ppm，48 h的半数致死浓度为0.66 ppm；刺五加碱(6)具有明显的杀虫活性，24 h的半数致死浓度为3.42 ppm。此外，还用结晶紫法研究了二花蛇舌草、毛花蛇舌草生物碱部位及分离的生物碱对MCF-7和HEPG-2细胞株的细胞毒活性。Anagyine(6)对MCF-7和HEPG-2癌细胞的IC50值分别为27.3+/-0.7和30.2+/-0.9微克/毫升。
Phytochemical investigation of Sophora secundiflora alkaloid fraction led to isolation of one new quinolizidine alkaloid (1) 13-methoxyanagyrine together with six known ones (2-7). The insecticidal activity of 70% methanol extract of leaves of S. secundiflora, S. tomentosa and the isolated alkaloids were assessed against 3(rd) instar larvae of Culex pipiens (Diptera: Culicidae) using different concentrations and mortality rate was recorded. Sophora tomentosa extract showed highest mortality rate with median lethal concentration LC50 3.11 ppm after 24 h and 0.66 ppm after 48 h and anagyrine (6) exhibited remarkably insecticidal activity with LC50 value of 3.42 ppm after 24 h of exposure. Additionally, cytotoxic activity of alkaloid fraction of S. secundiflora, S. tomentosa and isolated alkaloids was also studied using crystal violet assay against MCF-7 and HEPG-2 cell lines. Anagyrine (6) exhibited IC50 values of 27.3 +/- 0.7 and 30.2 +/- 0.9 mu g/mL against MCF-7 and HEPG-2 cancer cells, respectively.