Losing Reproduction: Effect of High Temperature on Female Biochemical Composition and Egg Quality in a Freshwater Crustacean with Direct Development, the Red Cherry Shrimp, Neocaridina davidi (Decapoda, Atyidae)
Losing Reproduction: Effect of High Temperature on Female Biochemical Composition and Egg Quality in a Freshwater Crustacean with Direct Development, the Red Cherry Shrimp, Neocaridina davidi (Decapoda, Atyidae)
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DOI：
10.1086/698266
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发表时间：
2018-06-01
影响因子：
1.6
通讯作者：
Tropea, Carolina
中科院分区：
文献类型：
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作者：
Balina, Sofia;Temperoni, Brenda;Tropea, Carolina
母体供给对于发育较短的无脊椎动物尤其重要，因为必须为发育中的胚胎提供大量的能量储备。在这种背景下，本研究的目的是分析直接发育的水生无脊椎动物中温度对雌性生化成分和成熟卵巢储备分配的影响，而成熟卵巢决定卵子质量。十足目甲壳类淡水虾 Neocaridina davidi 被用作实验模型。将刚孵化的幼鱼暴露在28℃或33℃下。在28℃下雌鱼显示出成熟的卵巢并产卵（对照产卵雌鱼），但在33℃下没有发现产卵雌鱼。200天后，33℃下的一半雌鱼转移到28℃，在那里它们迅速显示出成熟的卵巢并产卵（转移的产卵雌鱼）。处死产卵雌性和在28℃（对照非产卵雌性）和33℃（高温非产卵雌性）下未产卵的雌性以确定其生化成分。还分析了来自转移和对照产卵雌性的卵的数量、体积、重量和生化成分，作为其质量指标。雌性生化成分不受温度影响，因为对照和高温非产卵雌性具有相似的脂质、蛋白质和糖原含量。然而，卵巢成熟和产卵在 33 摄氏度时受到抑制，这表明该温度对营养物质转移到卵母细胞有负面影响。当雌性被移至 28 摄氏度后，这种效应迅速逆转；对照和转移产卵雌性的卵质量相似，只是后者的蛋白质含量较低。目前的结果为热应激下的繁殖储备分配提供了有价值的信息。
Maternal provisioning is particularly important in invertebrates with abbreviated development because large energy reserves must be provided for the developing embryo. In this context, the objective of the present study was to analyze in an aquatic invertebrate with direct development the effect of temperature on female biochemical composition and reserve allocation to maturing ovaries, which determine egg quality. A decapod crustacean, the freshwater shrimp Neocaridina davidi, was used as experimental model. Newly hatched juveniles were exposed to 28 degrees C or 33 degrees C. Females showed mature ovaries and spawned at 28 degrees C (control ovigerous females), but no ovigerous female was found at 33 degrees C. After a 200-day period, half of the females at 33 degrees C were transferred to 28 degrees C, where they rapidly showed mature ovaries and spawned (transferred ovigerous females). Ovigerous females and females that did not spawn at 28 degrees C (control non-ovigerous females) and at 33 degrees C (high-temperature non-ovigerous females) were sacrificed to determine their biochemical composition. The number, volume, weight, and biochemical composition of the eggs from transferred and control ovigerous females were also analyzed as indicators of their quality. Female biochemical composition was not influenced by temperature, because control and high-temperature non-ovigerous females had similar lipid, protein, and glycogen contents. However, ovarian maturation and spawning were inhibited at 33 degrees C, which indicates a negative effect of this temperature on nutrient transfer to the oocytes. This effect was rapidly reversed after females were moved to 28 degrees C; the eggs from control and transferred ovigerous females were of similar quality, except for a lower protein content in the latter. The present results provide valuable information on reserve allocation to reproduction under thermal stress.