Comparative genomics of insect juvenile hormone biosynthesis
Comparative genomics of insect juvenile hormone biosynthesis
复制标题
DOI：
10.1016/j.ibmb.2006.01.013
复制
发表时间：
2006-04-01
影响因子：
3.8
通讯作者：
Feyereisen, R
中科院分区：
文献类型：
--
作者：
Noriega, FG;Ribeiro, JMC;Feyereisen, R
昆虫保幼激素（JH）的生物合成及其神经内分泌调控是害虫和病媒化学防治的重要靶点。为了促进JH生物合成的分子研究，我们分析了ESTs从腺体产生JH，咽侧体（CA）的蟑螂Diploptera punctata，长期用作生理模式物种的昆虫，并比较它们与ESTs从CA的蚊子埃及伊蚊和按蚊albimanus。根据预测基因的可能功能，用基因本体分类法进行分析，并与果蝇和冈比亚按蚊基因进行比较。在蟑螂和蚊子CA的cDNA文库中发现了大量的相互匹配，这些匹配定义了已知的和怀疑的JH生物合成途径的酶，以及几个与信号转导相关的蛋白质，这些蛋白质可能在神经肽调节JH合成中起作用。在蟑螂和蚊子CA的同源物的小配体结合蛋白的昆虫，Takeout/JH结合蛋白和视黄醇结合蛋白的鉴定突出了迄今为止未被怀疑的代谢物贩运的复杂性，也许JH前体贩运，在这些内分泌腺。此外，许多功能未知的基因的相互匹配可能为深入研究allatal特异性细胞生理学提供了肥沃的土壤。(c)2006爱思唯尔有限公司保留所有权利。
The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular study of JH biosynthesis, we analyzed ESTs from the glands producing JH, the corpora allata (CA) in the cockroach Diploptera punctata, an insect long used as a physiological model species and compared them with ESTs from the CA of the mosquitoes Aedes aegypti and Anopheles albimanus. The predicted genes were analyzed according to their probable functions with the Gene Ontology classification, and compared to Drosophila and Anopheles gambiae genes. A large number of reciprocal matches in the cDNA libraries of cockroach and mosquito CA were found. These matches defined known and suspected enzymes of the JH biosynthetic pathway, but also several proteins associated with signal transduction that might play a role in the modulation of JH synthesis by neuropeptides. The identification in both cockroach and mosquito CA of homologs of the small ligand binding proteins from insects, Takeout/JH binding protein and retinol-binding protein highlights a hitherto unsuspected complexity of metabolite trafficking, perhaps JH precursor trafficking, in these endocrine glands. Furthermore, many reciprocal matches for genes of unknown function may provide a fertile ground for an in-depth study of allatal-specific cell physiology. (c) 2006 Elsevier Ltd. All rights reserved.