THE ONTOGENY OF EXCITATORY AMINO-ACID RECEPTORS IN RAT FOREBRAIN .1. N-METHYL-D-ASPARTATE AND QUISQUALATE RECEPTORS
THE ONTOGENY OF EXCITATORY AMINO-ACID RECEPTORS IN RAT FOREBRAIN .1. N-METHYL-D-ASPARTATE AND QUISQUALATE RECEPTORS
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DOI：
10.1016/0306-4522(90)90117-m
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
GELHARD, RE
中科院分区：
文献类型：
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作者：
INSEL, TR;MILLER, LP;GELHARD, RE
用体外受体放射自显影技术定量研究了大鼠前脑N-甲基-D-天冬氨酸和使君子酸受体与放射性配体结合的个体发生。出生后第1天在海马和纹状体中可以检测到与这两种受体的特异性结合。成人模式的结合N-甲基-D-天冬氨酸受体出生后第14天出现高密度的结合在CA 1（层oriens和层radiatum），齿状回（分子层）和纹状体（尾壳核）。在发育过程中，与额叶皮层外层的结合比成人水平高出45%。[3 H]氨基-3-羟基-5-甲基异恶唑-4-丙酸与使君子酸受体的结合在发育过程中表现出类似的过冲，但也主要表现出独特的分布，其中CA 3和杏仁核内侧部分表现出短暂的强烈标记。与[3 H]氨基-3-羟基-5-甲基异恶唑-4-丙酸的匀浆结合研究表明，与成人水平相比，出生后第21天全脑中使君子酸受体增加了73%。在发育过程中，兴奋性氨基酸与使君子酸位点结合的选择性与成年脑中的选择性相似。这些数据与其他最近的报告，表明使君子酸受体可能有一个在发展中的作用，不同于他们在成人大脑中的功能。
The ontogeny of radioligand binding to N-methyl-D-aspartate and quisqualate receptors in rat forebrain was studied quantitatively using in vitro receptor autoradiography. Specific binding to both receptors could be detected by postnatal day 1 in hippocampus and striatum. The adult pattern of binding to N-methyl-D-aspartate receptors emerged by postnatal day 14 with high densities of binding in CA1 (stratum oriens and stratum radiatum), dentate gyrus (molecular layer) and striatum (caudate-putamen). Binding to the outer laminae of frontal cortex was as much as 45% above adult levels during development. Binding of [3H]amino-3-hydroxy-5-methylisoxazole-4-proprionic acid to quisqualate receptors showed a similar overshoot during development, but also mainfested a unique distribution with CA3 and medial aspects of the amygdala exhibiting transient, intense labeling. Homogenate binding studies with [3H]amino-3-hydroxy-5-methylisoxazole-4-proprionic acid demonstrated a 73% increase in quisqualate receptors in whole brain at postnatal day 21 compared with adult levels. The selectivity of excitatory amino acid binding to the quisqualate site in development was similar to the selectivity in adult brain. These data taken with other recent reports, suggest that quisqualate receptors may have a role in development distinct from their function in the adult brain.