Unique host iron utilization mechanisms of Helicobacter pylori revealed with iron-deficient chemically defined media.
Unique host iron utilization mechanisms of Helicobacter pylori revealed with iron-deficient chemically defined media.
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缺铁化学成分确定的培养基揭示了幽门螺杆菌独特的宿主铁利用机制。
DOI：
10.1128/iai.01258-09
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发表时间：
2010
影响因子：
3.1
通讯作者：
Testerman,TraciL
中科院分区：
文献类型：
--
作者：
Senkovich,Olga;Ceaser,Shantelle;McGee,DavidJ;Testerman,TraciL
幽门螺杆菌长期感染胃粘膜，可以在粘液中、附着在细胞上和细胞内发现游离的幽门螺杆菌。幽门螺杆菌的生长需要铁，但体内使用的铁的来源尚不清楚。在以前的研究中，无法培养H.没有血清的幽门螺杆菌很难确定幽门螺杆菌可能使用哪些宿主铁源。幽门螺杆菌。使用缺铁、化学成分确定的培养基，我们确定了 H.幽门螺旋杆菌结合并从血红蛋白、转铁蛋白和乳铁蛋白中提取铁。与之前的报道相反，幽门螺旋杆菌同时使用牛和人的乳铁蛋白和转铁蛋白。与其他病原体不同，H.幽门优先结合无铁形式的转铁蛋白和乳铁蛋白，这限制了其从铁不饱和的正常血清中提取铁的能力。这种新策略可能已经发展到允许宿主组织在持续定殖期间有限生长，同时防止过度损伤或铁耗竭。
Helicobacter pylorichronically infects the gastric mucosa, where it can be found free in mucus, attached to cells, and intracellularly.H. pylorirequires iron for growth, but the sources of iron usedin vivoare unclear. In previous studies, the inability to cultureH. pyloriwithout serum made it difficult to determine which host iron sources might be used byH. pylori. Using iron-deficient, chemically defined medium, we determined thatH. pylorican bind and extract iron from hemoglobin, transferrin, and lactoferrin.H. pylorican use both bovine and human versions of both lactoferrin and transferrin, contrary to previous reports. Unlike other pathogens,H. pyloripreferentially binds the iron-free forms of transferrin and lactoferrin, which limits its ability to extract iron from normal serum, which is not iron saturated. This novel strategy may have evolved to permit limited growth in host tissue during persistent colonization while excessive injury or iron depletion is prevented.