Factors affecting in vitro maturation of minke whale (Balaenoptera acutorostrata) follicular oocytes
Factors affecting in vitro maturation of minke whale (Balaenoptera acutorostrata) follicular oocytes
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DOI：
10.1095/biolreprod56.2.523
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发表时间：
1997-02-01
影响因子：
3.6
通讯作者：
Ohsumi, S
中科院分区：
文献类型：
--
作者：
Fukui, Y;Mogoe, T;Ohsumi, S
研究了影响小须鲸卵泡卵母细胞体外成熟的因素。在实验1中，来自不同大小卵泡（小，1-5 mm;中等，6-10 mm;大，大于或等于11 mm）的卵母细胞的回收率在未成熟（54.7%）和成熟（53.5%）女性中相似，卵泡大小不影响回收率。在未成熟（55.5%）和成熟（52.1%）的鲸鱼中，大约有一半的卵母细胞被至少几层卵丘细胞包围。在培养前，71.7%和61.2%的卵母细胞从未成熟和成熟的鲸鱼，分别处于生殖囊阶段。对于IVM，研究了血清类型、激素和额外的卵丘细胞（实验2）的影响以及培养持续时间（24-120 h，实验3）的影响。实验2中研究的三个因素不影响成熟率。TCM 199+胎鲸血清+激素+卵丘细胞组的卵母细胞成熟率最高（21.6%），与只加胎牛血清组（6.6%）差异显著（P <0.05）。培养84 h后，观察到第一个具有挤出极体的卵母细胞。培养96 h时成熟率最高（27.3%），但培养90 h与120 h成熟率差异不显著。这些结果表明小须鲸未成熟卵泡卵母细胞的体外核成熟是可以被诱导的。
Factors affecting in vitro maturation (IVM) of minke whale (Balaenopetra acutorostrata) follicular oocytes were investigated. In experiment 1, recovery rates for oocytes from follicles of different sizes (small, 1-5 mm; medium, 6-10 mm; large, greater than or equal to 11 mm) were similar in both immature (54.7%) and mature (53.5%) females, and the follicular sizes did not affect recovery rate. Approximately half the oocytes recovered from small follicles in immature (55.5%) and mature (52.1%) whales were surrounded by at least a few layers of cumulus cells. Before culture, 71.7% and 61.2% of oocytes from immature and mature whales, respectively, were at the germinal vesicle stage. For IVM, effects of serum type, hormones, and additional cumulus cells (experiment 2) and effects of culture durations (24-120 h, experiment 3) were investigated. The three factors investigated in experiment 2 did not affect maturation rates. TCM199 supplemented with fetal whale serum, hormones, and additional cumulus cells showed the highest rate (21.6%) of matured oocytes and resulted in a significant difference from the rate in medium with only fetal calf serum added (6.6%). The first oocyte with an extruded polar body was observed after 84 h of culture. The maximum rate (27.3%) of matured oocytes was obtained by 96 h of culture, but there was no significant difference in the proportions of matured oocytes between 90 and 120 h in culture. These results indicate that in vitro nuclear maturation of immature follicular oocytes in minke whales can be induced.