Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli
Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli
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DOI：
10.1128/jb.186.10.3006-3014.2004
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发表时间：
2004-05-01
影响因子：
3.2
通讯作者：
Utsumi, R
中科院分区：
文献类型：
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作者：
Eguchi, Y;Okada, T;Utsumi, R
对大肠杆菌 EvgA/EvgS 双组分系统的组成型活性突变体进行转录分析，结果增强了 13 个 PhoP/PhoQ 调节基因的表达：crcA、hemL、mgtA、ompT、phoP、phoQ、proP、rstA、rstB、slyB、ybjG、yrbL 和 mgrB。两个系统之间的这种调节网络也是由于 EvgA 调节器生产过剩而出现的；然而，phoPQ 基因转录的增强并没有进一步激活 PhoP/PhoQ 调节基因的表达。这些结果证明了大肠杆菌中从 EvgA/EvgS 系统到 PhoP/PhoQ 系统的信号转导，并鉴定了需要这两个系统增强表达的基因。这是大肠杆菌中存在的复杂信号转导网络的一个例子。
Transcriptional analysis of a constitutively active mutant of the EvgA/EvgS two-component system of Escherichia coli resulted in enhanced expression of 13 PhoP/PhoQ-regulated genes, crcA, hemL, mgtA, ompT, phoP, phoQ, proP, rstA, rstB, slyB, ybjG, yrbL, and mgrB. This regulatory network between the two systems also occurred as a result of overproduction of the EvgA regulator; however, enhanced transcription of the phoPQ genes did not further activate expression of the PhoP/PhoQ-regulated genes. These results demonstrated signal transduction from the EvgA/EvgS system to the PhoP/PhoQ system in E. coli and also identified the genes that required the two systems for enhanced expression. This is one example of the intricate signal transduction networks that are posited to exist in E. coli.