Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins
Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins
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DOI：
10.1016/j.mito.2012.02.004
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发表时间：
2012-05-01
期刊：
影响因子：
4.4
通讯作者：
Kang, Dongchon
中科院分区：
文献类型：
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作者：
Matsumoto, Shinya;Uchiumi, Takeshi;Kang, Dongchon
线粒体氧化磷酸化（OXPHOS）蛋白由核DNA和线粒体DNA编码。核编码的OXPHOS mRNA具有特定的亚细胞定位，但对线粒体附近的定位知之甚少。在这里，我们比较了mRNA的结合多核糖体组分与那些在胞质，自由多核糖体组分。编码插入内膜的疏水OXPHOS蛋白的mRNA位于线粒体附近。相反，OXPHOS基因的mRNA主要定位于胞浆中，其跨膜结构域少于一个。RNA结合蛋白Y-box结合蛋白-1位于线粒体外膜并与OXPHOS mRNA结合。我们的发现为人类细胞中线粒体共翻译输入提供了新的见解。(C)2012 Elsevier B. V.和线粒体研究学会。All rights reserved.
The mitochondrial oxidative phosphorylation (OXPHOS) proteins are encoded by both nuclear and mitochondrial DNA. The nuclear-encoded OXPHOS mRNAs have specific subcellular localizations, but little is known about which localize near mitochondria. Here, we compared mRNAs in mitochondria-bound polysome fractions with those in cytosolic, free polysome fractions. mRNAs encoding hydrophobic OXPHOS proteins, which insert into the inner membrane, were localized near mitochondria. Conversely, OXPHOS gene which mRNAs were predominantly localized in cytosol had less than one transmembrane domain. The RNA-binding protein Y-box binding protein-1 is localized at the mitochondrial outer membrane and bound to the OXPHOS mRNAs. Our findings offer new insight into mitochondrial co-translational import in human cells. (C) 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.