Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity.
Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity.
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Cybr，一种细胞因子诱导蛋白，可结合 cytohesin-1 并调节其活性。
DOI：
10.1073/pnas.052712999
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发表时间：
2002
影响因子：
11.1
通讯作者：
Gadina,Massimo
中科院分区：
文献类型：
--
作者：
Tang,Pingtao;Cheng,TammyP;Agnello,Davide;Wu,Chang-You;Hissong,BruceD;Watford,WendyT;Ahn,Hyun-Jong;Galon,Jerome;Moss,Joel;Vaughan,Martha;O'Shea,JohnJ;Gadina,Massimo
细胞因子调节淋巴细胞的发育和分化，但它们如何精确地控制这些过程仍然知之甚少。通过使用微阵列技术检测甘氨酸诱导的基因，我们确定了编码Cybr的cDNA，其在与IL-2和IL-12孵育的细胞中显著增加。该mRNA在造血细胞和组织中含量最高。预测的氨基酸序列类似于GRP-1相关蛋白（GRASP），最近确定的视黄酸诱导的细胞粘连素结合蛋白。物理相互作用，依赖于卷曲螺旋结构域的Cybr和cytohesin-1，证明了从293 T细胞的过表达蛋白的共免疫沉淀。除了在细胞粘附中的作用外，细胞粘连素-1还是ARF GTP酶的鸟嘌呤核苷酸交换蛋白激活剂。Cybr能促进细胞粘连素1与ARF的5′-O-硫代三磷酸鸟苷结合。由于结合蛋白修饰的ADP核糖基化因子的激活细胞粘连素-1，我们指定这种甘氨酸诱导蛋白Cybr（细胞粘连素结合剂和调节剂）。
Cytokines regulate lymphocyte development and differentiation, but precisely how they control these processes is still poorly understood. By using microarray technology to detect cytokine-induced genes, we identified a cDNA encoding Cybr, which was increased markedly in cells incubated with IL-2 and IL-12. The mRNA was most abundant in hematopoietic cells and tissues. The predicted amino acid sequence is similar to that of GRP-1-associated protein (GRASP), a recently identified retinoic acid-induced cytohesin-binding protein. Physical interaction, dependent on the coiled-coil domains of Cybr and cytohesin-1, was demonstrated by coimmunoprecipitation of the overexpressed proteins from 293T cells. Cytohesin-1, in addition to its role in cell adhesion, is a guanine nucleotide-exchange protein activator of ARF GTPases. Acceleration of guanosine 5′-O-(thiotriphosphate) binding to ARF by cytohesin-1in vitrowas enhanced by Cybr. Because the binding protein modified activation of ADP ribosylation factor by cytohesin-1, we designate this cytokine-inducible protein Cybr (cytohesin binder and regulator).