RNA Controls PolyQ Protein Phase Transitions.
RNA Controls PolyQ Protein Phase Transitions.
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RNA控制Polyq蛋白相变。
DOI：
10.1016/j.molcel.2015.09.017
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发表时间：
2015-10-15
期刊：
影响因子：
16
通讯作者：
Gladfelter AS
中科院分区：
文献类型：
--
作者：
Zhang H;Elbaum-Garfinkle S;Langdon EM;Taylor N;Occhipinti P;Bridges AA;Brangwynne CP;Gladfelter AS
细胞中的区室化对于生物化学的空间和时间控制至关重要。除了膜结合的细胞器外，无膜区室在细胞中形成分区。越来越多的证据表明，这些隔间组装通过液-液相分离。然而，他们的组装时空控制，以及他们如何保持独特的功能和物理身份是知之甚少。我们以前已经显示了一个RNA结合蛋白与polyQ-扩展称为Whi 3是必不可少的空间模式的细胞周期蛋白和cytosol转录。在这里，我们表明，特定的mRNA是已知的Whi 3驱动相分离的生理目标。mRNA可以改变液滴的粘度、它们融合的倾向以及组分与本体溶液的交换速率。不同的mRNA赋予液滴不同的生物物理性质，表明mRNA可以为组装体带来个性。我们的研究结果表明，mRNA不仅可以编码遗传信息，而且还可以编码相分离区室的生物物理特性。
Compartmentalization in cells is central to the spatial and temporal control of biochemistry. In addition to membrane-bound organelles, membrane-less compartments form partitions in cells. Increasing evidence suggests that these compartments assemble through liquid-liquid phase separation. However the spatiotemporal control of their assembly, and how they maintain distinct functional and physical identities is poorly understood. We have previously shown an RNA-binding protein with a polyQ-expansion called Whi3 is essential for the spatial patterning of cyclin and formin transcripts in cytosol. Here, we show that specific mRNAs that are known physiological targets of Whi3 drive phase separation. mRNA can alter the viscosity of droplets, their propensity to fuse, and the exchange rates of components with bulk solution. Different mRNAs impart distinct biophysical properties of droplets indicating mRNA can bring individuality to assemblies. Our findings suggest that mRNAs can encode not only genetic information, but also the biophysical properties of phase-separated compartments.