Overexpression of metastasis-associated MTA1 in oral squamous cell carcinomas: correlation with metastasis and invasion
Overexpression of metastasis-associated MTA1 in oral squamous cell carcinomas: correlation with metastasis and invasion
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DOI：
10.1016/j.ijom.2008.05.020
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发表时间：
2008-11-01
影响因子：
2.4
通讯作者：
Mizuno, A.
中科院分区：
文献类型：
--
作者：
Kawasaki, G.;Yanamoto, S.;Mizuno, A.
转移相关蛋白1（MTA 1）在人体组织中以低水平生理表达。它的表达与实体的进展有关，并且在癌细胞系中很常见。本研究旨在探讨VITA 1在鳞状细胞癌（SCC）中的表达及其作为转移标志物的可能性。用抗NITA 1多克隆抗体对38例口腔鳞状细胞癌标本进行抗生物素蛋白-过氧化物酶染色。对人鳞状细胞癌细胞系SAS、HSC 2、OSC 19和OSC 20的MTA 1 mRNA表达进行了分析。38例SCC组织中有33例检测到MTA 1蛋白表达。在组织学上，MTA 1蛋白的产生与癌细胞的侵袭密切相关，在临床上，MTA 1蛋白的产生与淋巴结转移相关。在癌细胞系中，HSC 2显示最低的mRNA表达，而OSC 20显示最高的MTA 1 mRNA表达。在Matrigel侵袭测定中，HSC 2细胞系显示最低的侵袭，而0 SC 20细胞系显示最高的侵袭。RNAi介导的MTA 1沉默可降低OSC 20细胞的侵袭指数。口腔鳞癌组织中MTA 1的表达可能与其侵袭能力增强有关，而侵袭能力的增强可能导致淋巴结转移。
Metastasis-associated protein 1 (MTA 1) is physiologically expressed at low levels in human tissues. Its expression is associated with progression of solid and is common in cancer cell lines. This study investigated whether VITA 1 was expressed ill squamous cell carcinoma (SCC) and would be a useful metastatic marker. Specimens from 38 patients with oral SCC were stained using the avidinbiotin-peroxidase technique With polyclonal antibodies against NITA 1. Human SCC cell lines SAS, HSC2, OSC19 and OSC20 were analysed for MTA1 mRNA expression.MTA1 expression ill control tissues Was significantly lower than in carcinomas. MTA1 protein expression was detected in 33 of 38 SCC tissues from patients. HIstologically, MTA 1 protein production was strongly associated with cancer cell invasion, and clinically there was a correlation between lymph node metastasis and MTA 1 production. Among the cancer cell lines, HSC2 showed the lowest mRNA expression, and OSC20 showed the highest MTA 1 mRNA expression. In the Matrigel invasion assay, the HSC2 cell line showed the lowest invasion and the OSC20 cell line showed the highest invasion. RNAi-mediated MTA1 silencing ill the OSC20 cells decreased the invasion index. MTA1 expression in oral SCC may be associated with increased invasive ability, which may cause lymph node metastasis.