Transforming growth factor beta 1 and metalloproteinase-9 overexpression in colorectal cancer (CC) and adenoma
Transforming growth factor beta 1 and metalloproteinase-9 overexpression in colorectal cancer (CC) and adenoma
复制标题
DOI：
10.1007/s00384-007-0296-9
复制
发表时间：
2007-10-01
影响因子：
2.8
通讯作者：
Malecka-Panas, Ewa
中科院分区：
文献类型：
--
作者：
Daniel, Piotr;Wagrowska-Danilewicz, Malgorzata;Malecka-Panas, Ewa
虽然转化生长因子β（TGF β）1和金属蛋白酶-9（MMP-9）在结直肠癌（CC）中的作用已被充分证明，但很少有证据支持其在早期癌发生中的作用。本研究的目的是确定TGF β 1、MMP-9和Ki-67在CC和腺瘤性息肉中的免疫组化表达模式，研究组包括50例大肠息肉和33例CC患者。内镜下切除的息肉和CC活检组织进行了评价与组织病理学检查和免疫组化。以30例健康体检者的活检组织为对照组。在62例腺瘤中，检出高度异型增生（HGD）33例，低度异型增生（LGD）29例。CC的平均TGF β 1、MMP-9和Ki-67 LI显著高于HGD息肉（分别为p < 0.01、0.01和0.01）。HGD息肉中平均TGF β 1、MMP-9和Ki-67 LI显著高于LGD息肉（分别为p < 0.01、0.01和0.01）。TGF β 1、MMP-9和Ki-67 LI在LGD组和对照组之间无统计学差异（分别为p > 0.05、0.05和0.05）。TGF β 1与MMP-9、Ki-67、MMP-9呈正相关（r=0.898、r=0.938、r=0.913）。TGF β 1、MMP-9、Ki-67 LI与结直肠腺瘤的临床指标无相关性，但TGF β 1、MMP-9在结直肠腺瘤中的表达与结直肠腺瘤的异型增生程度有关。我们认为TGF β 1、MMP-9的过度表达是结直肠癌发生的早期事件，可能具有预后价值。
Although the role of transforming growth factor beta (TGFbeta) 1 and metalloproteinase-9 (MMP-9) is well documented in colorectal cancer (CC), there is a little evidence supporting its role in early carcinogenesis. The aim of the study was to determine the pattern of immunohistochemical expression of TGFbeta1, MMP-9, and Ki-67 in CC and adenomatous polyps.The study group comprised 50 patients with colorectal polyps and 33 patients with CC. Endoscopically removed polyps and CC biopsies had been evaluated with histopatologic examination and immunohistochemistry. The biopsies from 30 healthy objects served as a control group. For all antibodies labeling indices (LI) had been calculated.Among 62 adenomas, 33 high-grade dysplasia (HGD) and 29 low-grade dysplasia (LGD) had been detected. Mean TGFbeta1, MMP-9, and Ki-67 LI in CC were significantly higher (p < 0.01, 0.01, and 0.01, respectively) than in HGD polyps. Mean TGFbeta1, MMP-9, and Ki-67 LI in HGD polyps were significantly higher than in LGD polyps (p < 0.01, 0.01, and 0.01, respectively). There had been no statistical difference in TGFbeta1, MMP-9, and Ki-67 LI between LGD and the control group (p > 0.05, 0.05, and 0.05, respectively). There was a positive correlation between TGFbeta1 and MMP-9 (r=0.898), Ki-67 and MMP-9 (r=0.938), and TGFbeta1 and Ki-67 (r=0.913). We did not observe any correlation between TGFbeta1, MMP-9, Ki-67 LI and the clinical parameters evaluated.The increased expression of TGFbeta1, MMP-9 observed in colorectal adenomas seems to be related to the grade of dysplasia. We assume that overexpression of TGFbeta1, MMP-9 represent an early event in colorectal carcinogenesis and may possibly have the prognostic value.