The placenta is a niche for hematopoietic stem cells
The placenta is a niche for hematopoietic stem cells
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DOI：
10.1016/j.devcel.2004.12.016
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发表时间：
2005-03-01
影响因子：
11.8
通讯作者：
Mikkola, HKA
中科院分区：
文献类型：
--
作者：
Gekas, C;Dieterlen-Lièvre, F;Mikkola, HKA
在胚胎发生期间，造血系统在时间上和解剖上受到限制的部位发育。最终HSC的解剖学起源尚未完全确定，并且对于不同的胎儿造血微环境如何指导HSC的发育知之甚少。在这里，我们证明了小鼠胎盘作为造血器官的功能，在中期孕育了大量的多能造血干细胞。胎盘中HSC活性的开始与从E10.5-E11.0开始的AGM（主动脉-性腺-中肾）区域相似。然而，胎盘HSC池扩大到E12.5-E13.5，包含的HSC比AGM多15倍。胎盘中CD34(+)c-kit(+) HSC库的扩增发生在胎肝中HSC初始扩增之前和期间。重要的是，胎盘HSC库不能用后来出现的罕见循环HSC来解释。这些数据支持胎盘在建立哺乳动物最终造血系统中的重要作用，但未被认识到。
The hematopoietic system develops during embryogenesis at temporally and anatomically restricted sites. The anatomical origin of definitive HSCs is not fully resolved, and little is known about how the different fetal hematopoietic microenvironments direct HSC development. Here, we show that the mouse placenta functions as a hematopoietic organ that harbors a large pool of pluripotent HSCs during midge-station. The onset of HSC activity in the placenta parallels that of the AGM (aorta-gonad-mesonephros) region starting at E10.5-E11.0. However, the placental HSC pool expands until E12.5-E13.5 and contains > 15-fold more HSCs than the AGM. The expansion of the CD34(+)c-kit(+) HSC pool in the placenta occurs prior to and during the initial expansion of HSCs in the fetal liver. Importantly, the placental HSC pool is not explained by rare circulating HSCs, which appear later. These data support an important, but unappreciated, role for the placenta in establishing the mammalian definitive hematopoietic system.