The Relation between the Minor Components of Whole Normal Human Adult Hemoglobin as Isolated by Chromatography and Starch Block Electrophoresis
The Relation between the Minor Components of Whole Normal Human Adult Hemoglobin as Isolated by Chromatography and Starch Block Electrophoresis
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色谱和淀粉块电泳分离的正常成人血红蛋白微量成分之间的关系
DOI：
10.1021/ja01467a046
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发表时间：
1961
影响因子：
15
通讯作者：
W. Schroeder
中科院分区：
文献类型：
--
作者：
A. Schnek;W. Schroeder
用淀粉块电泳法可以从正常成人红细胞的溶血产物中分离出三种血红蛋白成分：主要成分Ai在移动最慢的成分A2和移动最快的成分A3之间的中间位置移动。通过色谱法，可以检测到八种血红蛋白组分：五种（Aia、Aib、Aic、Aid和Aie）比主要组分（An）更快地沿柱向下移动，两种（Ania和Amb）移动得更慢。也存在不含血红素的蛋白质。本研究确定了用两种方法分离的组分之间的对应性：A1含有Aic、Aid和Ai 8以及An; A2含有Anib和非血红素蛋白; A3含有Aia、Aib和非血红素蛋白。
Three hemoglobin components may be separated from hemolysates of normal adult red blood cells by starch block elec-trophoresis: the main component, Ai, moves at a position intermediate between the most slowly moving component, A2, and the most rapidly moving component, A3. By chromatography, eight hemoglobin components may be detected: five (Aia, Aib, Aic, Aid and Aie) move down the column more rapidly than the main component (An) and two move more slowly (Ania and Amb). Non-heme containing proteins are also present. The present investigation has determined the corre-spondence between the components as isolated by the two methods: Ai contains Aic, Aid and Ai8, and An; A2 contains Anib and non-heme protein (s); A3 contains Aia, Aib and non-heme protein (s).