SPONTANEOUS LIPID-PEROXIDATION IN RABBIT AND MOUSE EPIDIDYMAL SPERMATOZOA - DEPENDENCE OF RATE ON TEMPERATURE AND OXYGEN CONCENTRATION
SPONTANEOUS LIPID-PEROXIDATION IN RABBIT AND MOUSE EPIDIDYMAL SPERMATOZOA - DEPENDENCE OF RATE ON TEMPERATURE AND OXYGEN CONCENTRATION
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DOI：
10.1095/biolreprod32.2.342
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发表时间：
1985-01-01
影响因子：
3.6
通讯作者：
STOREY, BT
中科院分区：
文献类型：
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作者：
ALVAREZ, JG;STOREY, BT
自发脂质过氧化反应的速率，作为测量的丙二醛（MA）的形成，确定为O2浓度和温度的函数在小鼠和兔精子从附睾尾释放。过氧化速率在悬浮介质中的O2浓度至210 μ M（在34 ℃下环境空气的PO 2 [O2分压]下的浓度）是线性的。C）对于来自两个物种的精子，温度范围为34 - 40度。C.这是两种物质的反应可测量的范围：低于34 ℃。当温度高于40 ℃时，速率变得太慢而不能准确测量兔精子的速率。小鼠精子的速度变得太快。该窄范围是过氧化过程的高活化能（EA）的特征。从Kox与（T）-1的曲线图计算EA值，其中kox是二阶速率常数，单位为（108个细胞/ml）-1。它由以下等式定义：vma = kox（sp）（O2）是悬浮介质中的O2浓度。对于小鼠精子，EA为78.7 kcal/mol（329 KJ/mol）;对于兔精子，该值为77.6 kcal/ml（324 KJ/mol）。这些高Ea和随之而来的自发脂质过氧化速率对温度的急剧依赖性有利于附睾中的长精子寿命。32.degree. C和低PO 2，同时允许在37 ℃下相对较短的寿命。C组输卵管内PO_2较高。
The rate of spontaneous lipid peroxidation, as measured by formation of malonaldehyde (MA), was determined as a function of O2 concentration and temperature in mouse and rabbit spermatozoa released from the cauda epididymidis. The peroidation rate was linear in O2 concentration in the suspending medium up to 210 .mu.M (the concentration at PO2 [partial pressure of O2] of ambient air at 34.degree. C) for sperm from both species over the temperature range 34-40.degree. C. This is the range over which the reaction is measurable for both species: below 34.degree. C, the rates became too slow to be measured accurately for rabbit sperm, while above 40.degree. C the rates for mouse sperm become too rapid. This narrow range is characteristic of a high activation energy (EA) for the peroxidation process. Values of EA were calculated from plots of Kox vs. (T)-1 where kox is a 2nd order rate constant with the units (108 cells/ml)-1. It is defined by the equation: vma = kox (sp) (O2) is the O2 concentrations in the suspending medium. For mouse sperm, EA was 78.7 kcal/mol (329 KJ/mol); for rabbit sperm, the value was 77.6 kcal/ml (324 KJ/mol). These high Ea and consequent steep dependence of the spontaneous lipid peroxidation rates on temperature favor long sperm life in the epididymis is .apprx. 32.degree. C and low PO2 in these scrotal animals, while allowing for a relatively short life at 37.degree. C at higher PO2 in the oviduct.