BRAM1, a BMP receptor-associated molecule involved in BMP signalling
BRAM1, a BMP receptor-associated molecule involved in BMP signalling
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DOI：
10.1046/j.1365-2443.1998.00186.x
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发表时间：
1998-04-01
期刊：
影响因子：
2.1
通讯作者：
Shibuya, H
中科院分区：
文献类型：
--
作者：
Kurozumi, K;Nishita, M;Shibuya, H
背景资料：TGF-β超家族成员通过刺激丝氨酸/苏氨酸激酶受体引发信号。最近，与几种TGF-β家族受体相关的分子已被克隆。一种这样的分子，亲免素FKBP 12，已被报道与TGF-β家族I型受体相互作用。然而，与receptor.Results信号特异性分子相互作用的身份是unknown：为了澄清介导骨形态发生蛋白（BMP）受体信号的因素，与BMP IA型受体（BMPR-IA）的细胞质分子分离使用酵母双杂交系统。我们将分子BMP受体相关分子1（BRAM 1）命名为BRAM 1，BRAM 1是BS 69的一种选择性剪接形式，BS 69是一种先前被鉴定为腺病毒E1 A相关蛋白的因子。BRAM 1定位于哺乳动物细胞的细胞质区域，而BS 69定位于细胞核。BRAM 1在哺乳动物细胞中特异性结合BMPR-IA。结论：利用酵母双杂交系统分离得到BMPR-IA结合分子BRAM 1，该分子与BMPR-IA特异性结合。因此，BRAM 1可以作为BMP信号通路中的相互作用蛋白。
Background: TGF-beta superfamily members elicit signals through the stimulation of serine/threonine-kinase receptors, Recently, molecules associated with several TGF-beta family receptors have been cloned. One such molecule, the immunophilin FKBP12, has been reported to interact with TGF-beta family type I receptors. However, the identity of signalling specific molecules interacting with the receptor was unknown.Results: To clarify the factors mediating bone morphogenetic protein (BMP) receptor signalling, a cytoplasmic molecule associated with the BMP type IA receptor (BMPR-IA) was isolated using the yeast two-hybrid system. We designated the molecule BMP receptor associated molecule 1 (BRAM1), BRAM1 is an alternatively spliced form of BS69, a factor previously identified as an adenovirus E1A-associated protein. BRAM1 was localized to the cytoplasmic region in mammalian cells, whereas BS69 is localized to the nucleus. BRAM1 bound specifically to BMPR-IA in mammalian cells. The C-terminal half of BRAM1 was found to be sufficient for binding to BMPR-IA.Conclusions: BRAM1, a BMPR-IA associated molecule, was isolated using the yeast two-hybrid system, and found to associate specifically with BMPR-IA. BRAM1 may thus serve as an interacting protein in the BMP signal pathway.