Population study of sizes and components of self-reproducing giant multilamellar vesicles
Population study of sizes and components of self-reproducing giant multilamellar vesicles
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DOI：
10.1021/la703017s
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发表时间：
2008-04-01
期刊：
影响因子：
3.9
通讯作者：
Sugawara, Tadashi
中科院分区：
文献类型：
--
作者：
Toyota, Taro;Takakura, Katsuto;Sugawara, Tadashi
用流式细胞术对一个自我繁殖的巨型多层囊泡（gmv）系统进行了群体分析。来自每个GMV的前向光散射（FS）、侧光散射（SS）和荧光（FL）强度的多维分布提供了由10(4)个囊泡组成的种群变化的信息。FS-FL点阵图显示，加入膜前驱体后，新生成的囊泡大小分布与原膜基本相同，但催化剂含量减少。这一结果可以解释为gmv自我繁殖发生的证据。此外，当添加催化剂溶液到新的gmv中时，新的gmv将催化剂的量恢复到初始值，并保持其尺寸分布不变。这些结果首次证明了在它们的自我繁殖周期中GMV大小分布的新现象。
Population analysis of a system of self-reproducing giant multilamellar vesicles (GMVs) was carried out by means of flow cytometry. The multidimensional distribution of forward light scattering (FS), side light scattering (SS), and fluorescence (FL) intensities originating from each GMV provided information about changes in a population composed of 10(4) vesicles. FS-FL dot plots indicated that, after the addition of the membrane precursor, the size distribution of the newly generated vesicles was nearly the same as that of the original, but the catalyst content was reduced. This result can be interpreted as evidence for the occurrence of the self-reproduction of GMVs. Moreover, the new GMVs recovered the amount of catalyst to the initial value, keeping their size distribution constant, when a solution of the catalyst was added to the new GMVs. These results are the first experimental evidence for a novel phenomenon on GMV size distribution during their self-reproducing cycle.