A small family of elements with long inverted repeats is located near sites of developmentally regulated DNA rearrangement in Tetrahymena thermophila.
A small family of elements with long inverted repeats is located near sites of developmentally regulated DNA rearrangement in Tetrahymena thermophila.
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一个具有长反向重复序列的小元件家族位于嗜热四膜虫发育调控 DNA 重排位点附近。
DOI：
10.1128/mcb.14.9.5939-5949.1994
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发表时间：
1994
影响因子：
5.3
通讯作者：
Karrer,KM
中科院分区：
文献类型：
--
作者：
Wells,JM;Ellingson,JL;Catt,DM;Berger,PJ;Karrer,KM
在纤毛虫的胚系微核发育过程中，广泛的DNA重排发生在体细胞大核发育过程中。嗜热四膜虫Tlr1的微核连接和发育调控DNA重排的大核产物已被克隆。染色体内重排将微核中相隔13kb以上的序列连接在一起，消除了适度重复的微核特异DNA序列。在微核连接附近有一个长达825个碱基的反向重复序列。反向重复序列包含两个不同的19个碱基的串联重复序列。19个碱基的重复序列相互关联，并与微核基因组中七个位置的DNA重排有关。Southern印迹分析与较大重复序列对中19-bp重复序列的出现一致。另一名家庭成员被隔离。克隆中19-MERS也非常接近重排连接。我们建议19-MERS定义了一个发育调节的DNA重排的小家族，这些重排的元件在连接点附近具有长的反向重复序列。我们讨论了转座元件通过捕获基本细胞功能所需的分子机制而进化的可能性。
Extensive DNA rearrangement occurs during the development of the somatic macronucleus from the germ line micronucleus in ciliated protozoans. The micronuclear junctions and the macronuclear product of a developmentally regulated DNA rearrangement inTetrahymena thermophila, Tlr1, have been cloned. The intrachromosomal rearrangement joins sequences that are separated by more than 13 kb in the micronucleus with the elimination of moderately repeated micronucleus-specific DNA sequences. There is a long, 825-bp, inverted repeat near the micronuclear junctions. The inverted repeat contains two different 19-bp tandem repeats. The 19-bp repeats are associated with each other and with DNA rearrangements at seven locations in the micronuclear genome. Southern blot analysis is consistent with the occurrence of the 19-bp repeats within pairs of larger repeated sequences. Another family member was isolated. The 19-mers in that clone are also in close proximity to a rearrangement junction. We propose that the 19-mers define a small family of developmentally regulated DNA rearrangements having elements with long inverted repeats near the junction sites. We discuss the possibility that transposable elements evolve by capture of molecular machinery required for essential cellular functions.