Molecular and Genetic Analyses of the Putative Proteus O Antigen Gene Locus
Molecular and Genetic Analyses of the Putative Proteus O Antigen Gene Locus
复制标题
假定变形杆菌 O 抗原基因座的分子和遗传学分析
DOI：
10.1128/aem.02946-09
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发表时间：
2010-08-01
影响因子：
4.4
通讯作者：
Wang, Lei
中科院分区：
文献类型：
--
作者：
Wang, Quan;Torzewska, Agnieszka;Wang, Lei
变形杆菌是一种典型的机会致病菌，主要与人类尿路感染(UTI)有关。变形杆菌O抗原是细胞表面变化最大的成分之一，O抗原异质性被用于变形杆菌分离株的血清学分类。尽管大多数变形杆菌的O抗原结构已被鉴定，但O抗原基因座仍未得到很好的描述。本研究利用聚合酶链式反应-限制性片段长度多态(RFLP)对40株变形杆菌的序列进行比较，确定了变形杆菌O抗原基因座，并证明了该区域的高度异质性。这一分析确定了5个可能的变形杆菌O抗原基因簇，并根据它们与现有数据库中基因的相似性提出了这些O抗原相关基因的可能功能。最后，通过筛选属于68个变形杆菌O抗原血清组的79株菌株，从这五个血清组中鉴定出变形杆菌特异性基因。据我们所知，这是第一次推测变形杆菌O抗原基因座的分子特征，我们描述了一种新的分子分类方法来鉴定不同的变形杆菌血清群。
Proteus species are well-characterized opportunistic pathogens primarily associated with urinary tract infections (UTI) of humans. The Proteus O antigen is one of the most variable constituents of the cell surface, and O antigen heterogeneity is used for serological classification of Proteus isolates. Even though most Proteus O antigen structures have been identified, the O antigen locus has not been well characterized. In this study, we identified the putative Proteus O antigen locus and demonstrated this region's high degree of heterogeneity by comparing sequences of 40 Proteus isolates using PCR-restriction fragment length polymorphism (RFLP). This analysis identified five putative Proteus O antigen gene clusters, and the probable functions of these O antigen-related genes were proposed, based on their similarity to genes in the available databases. Finally, Proteus-specific genes from these five serogroups were identified by screening 79 strains belonging to the 68 Proteus O antigen serogroups. To our knowledge, this is the first molecular characterization of the putative Proteus O antigen locus, and we describe a novel molecular classification method for the identification of different Proteus serogroups.