The P gene of Newcastle disease virus does not encode an accessory X protein
The P gene of Newcastle disease virus does not encode an accessory X protein
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DOI：
10.1099/vir.0.80160-0
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发表时间：
2004-08-01
影响因子：
3.8
通讯作者：
de Leeuw, O
中科院分区：
文献类型：
--
作者：
Peeters, B;Verbruggen, P;de Leeuw, O
许多副粘病毒编码非必需辅助蛋白，这些蛋白参与病毒复制的调节和细胞抗病毒反应的抑制。有人提出，新城疫病毒 (NDV) 的 P 基因 mRNA 通过在第 120 位保守的框内 AUG 密码子处翻译起始，编码一种辅助蛋白 - 所谓的 X 蛋白。 使用特异性检测 P 和 X 蛋白的单克隆抗体，结果表明辅助 X 蛋白在 NDV 感染的细胞中不表达。 AUG 变为 GCC (Ala) 或 GUC (Val) 密码子的重组新城疫病毒株是可行的，但显示毒力降低，可能是因为氨基酸变化影响了 P 和/或 V 蛋白的功能。
Many paramyxoviruses encode non-essential accessory proteins that are involved in the regulation of virus replication and inhibition of cellular antiviral responses. It has been suggested that the P gene mRNA of Newcastle disease virus (NDV) encodes an accessory protein - the so-called X protein - by translation initiation at a conserved in-frame AUG codon at position 120. Using a monoclonal antibody that specifically detected the P and X proteins, it was shown that an accessory X protein was not expressed in NDV-infected cells. Recombinant NDV strains in which the AUG was changed into a GCC (Ala) or GUC (Val) codon were viable but showed a reduction in virulence, probably because the amino acid change affected the function of the P and/or V protein.