Application of immunohistochemistry and in situ hybridization for detection of bovine coronavirus in paraffin-embedded, formalin-fixed intestines
Application of immunohistochemistry and in situ hybridization for detection of bovine coronavirus in paraffin-embedded, formalin-fixed intestines
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免疫组织化学和原位杂交在石蜡包埋、福尔马林固定肠中检测牛冠状病毒的应用
DOI:
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发表时间:
1997
影响因子:
9.4
通讯作者:
S. Kapil
中科院分区:
文献类型:
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作者:
Z. Zhang;G A Andrews;C. Chard;H C Minocha;S. Kapil
A monoclonal antibody (MAb) (Z3A5) against spike protein subunit of bovine coronavirus (BCV) reacted with the virus in formalin-fixed intestines in an immunoperoxidase test. We found an 88% correlation between immunohistochemistry with Z3A5 and in situ hybridization with a BCV nucleoprotein cDNA probe. MAb Z3A5 reacted with 90 BCV isolates from the United States and was an effective reagent for the diagnosis of BCV.
DOI:
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发表时间:
1991
期刊:
Laboratory investigation; a journal of technical methods and pathology
影响因子:
--
作者:
Brownstein,DG;Smith,AL;Jacoby,RO;Johnson,EA;Hansen,G;Tattersall,P
通讯作者:
Tattersall,P