Effects of therolABC, rolAB, and CaMV 35S-rolC genes on growth and nitrogen fixation inLotus corniculatus L.
Effects of therolABC, rolAB, and CaMV 35S-rolC genes on growth and nitrogen fixation inLotus corniculatus L.
复制标题
therolABC、rolAB和CaMV 35S-rolC基因对百脉根生长和固氮的影响。
DOI：
10.1007/bf02908825
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发表时间：
1995
影响因子：
1.5
通讯作者：
I. Macháčková
中科院分区：
文献类型：
--
作者：
D. Požárková;G. Šiffelová;V. Našinec;I. Macháčková
利用根癌农杆菌ABC、ro AB和35S-ro构建法对莲花进行了遗传转化。对筛选出的转化子克隆后代的生长、地上部和根部形态、地上部细胞分裂素和生长素浓度、叶片中叶绿素含量、结瘤和根瘤固氮酶活性与对照植株进行了比较。与AB、ROABC和对照相比，35S-roll转化体发生了最显著的变化(矮化、地上部和根部分枝、节间缩短、根瘤形成延迟、固氮酶活性降低、叶片色素含量减少)。ABC转化子在形态上以及测量的大多数其他性状上都与对照植物非常相似。
Lotus corniculatushas been transformed byAgrobacterium tumefaciensharbouring therolABC, rolAB, and 35S-rolCconstructs. Growth, shoot and root morphology, cytokinin and auxin concentrations in the shoots, chlorophyll content in the leaves, nodulation and nodule nitrogenase activity of clonal progenies of selected transformants, in which the transferredrolsequences were proved, were compared with control plants.The most striking alterations were observed in the 35S-rolCtransformants (dwarfism, shoot and root branching, shorter internodes, delayed nodule formation, decreased nitrogenase activity, reduced content of leaf pigments) in comparison with either therolAB, rolABC, or controls. TherolABandrolABCtransformants were very similar to the control plants in their morphology as well as in most other traits measured.