S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea
S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea
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DOI：
10.1007/s00705-003-0225-3
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发表时间：
2004-03-01
影响因子：
2.7
通讯作者：
Kwon, HM
中科院分区：
文献类型：
--
作者：
Lee, SK;Sung, HW;Kwon, HM
2001年至2002年间，从韩国疑似传染性支气管炎鸡的肾脏、气管和盲肠扁桃体中分离到15株传染性支气管炎病毒（IBV）分离株。应用逆转录-聚合酶链反应（RT-PCR）扩增IBV分离株S1糖蛋白基因，并进行限制性片段长度多态性（RFLP）分析。用限制性内切酶Hae Ⅲ、BstYI和Xcm Ⅰ将15株韩国IBV分离株的RFLP图谱分为4组。15个分离株中有3个、1个和1个的RFLP图谱分别与IBV阿肯色州、康涅狄格州和马萨诸塞州株的图谱相对应。15个分离株中有10个产生了独特的KM 91 RFLP图谱，这在以前在韩国分离的IBV KM 91株中观察到。为了确认IBV分离株S1基因的遗传多样性，对15株IBV分离株中的9株代表性病毒RNA进行扩增、克隆、测序，并与非韩国IBV株的已发表序列进行比较。韩国IBV分离株氨基酸序列同源性为61.8%，（K446-01和K161-02）和96.1%（K281-01和K210-02）的氨基酸序列同源性为42.9%，与非韩国IBV株相比，K161-02和GA 980470的感染率为96.5%（K203-02和KB 8523）。通过系统发育树分析，韩国IBV野外分离株分为5个簇，其中3个簇与非韩国IBV株不同。特别是韩国IBV分离株K 069 -01、K507-01、K774-01和K142-02形成单独的簇。看来，IBV继续演变和IBV显示各种遗传差异可能在韩国共同流通。
Fifteen isolates of Infectious bronchitis virus (IBV) were obtained from the kidney, trachea, and cecal tonsil of IB suspected chickens between 2001 and 2002 years in Korea. The S1 glycoprotein gene of IBV isolates were amplified by reverse transcriptase - polymerase chain reaction (RT-PCR) and analyzed by restriction fragment length polymorphism (RFLP) analysis. Fifteen Korean IBV isolates were classified into 4 groups by their RFLP patterns using restriction enzymes, HaeIII, BstYI, and XcmI. The RFLP patterns for 3, 1, and 1 of 15 isolates corresponded to the patterns of IBV Arkansas, Connecticut, and Massachusetts strains, respectively. Ten of 15 isolates generated unique KM91 RFLP pattern that was observed in the IBV KM 91 strain previously isolated in Korea. To confirm genetic diversity in the S1 genes of IBV isolates, viral RNAs of representative 9 of 15 IBV isolates were amplified, cloned, sequenced and compared with published sequences for non-Korean IBV strains. Korean IBV isolates showed amino acid sequence similarity between 61.8% (K446-01 and K161-02) and 96.1% (K281-01 and K210-02) with each other and they showed amino acid sequence similarity between 42.9% (K161-02 and GA980470) and 96.5% (K203-02 and KB8523) compared to non-Korean IBV strains. By phylogenetic tree analysis, Korean IBV field isolates were branched into five clusters in which 3 clusters were differentiated from non-Korean IBV strains. Especially, Korean IBV isolates K069-01, K507-01, K774-01 and K142-02 formed a separate cluster. It seems that IBVs continue to evolve and IBVs showing various genetic differences may cocirculate in Korea.