De novo transcriptome sequencing and comparative analysis to discover genes involved in ovarian maturity in Strongylocentrotus nudus
De novo transcriptome sequencing and comparative analysis to discover genes involved in ovarian maturity in Strongylocentrotus nudus
复制标题
从头转录组测序和比较分析发现裸甲鱼卵巢成熟相关基因
DOI：
10.1016/j.cbd.2017.05.002
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发表时间：
2017-09-01
影响因子：
3
通讯作者：
Liu, Xiaolin
中科院分区：
文献类型：
--
作者：
Jia, Zhiying;Wang, Qiai;Liu, Xiaolin
Strongylocentrotus nudus 是一种食用海胆，主要产自中国。相关研究表明，直径较大的裸鳅性腺早熟，成熟过程延长，上市时间较长，适口性较好。然而，这种现象背后的分子机制仍不清楚。在这里，应用转录组测序来研究不同壳直径的成体S. nudus的卵巢，以探索可能的机制。在本研究中，使用 HiSeq (TM) 2500 平台构建了四个独立的 cDNA 文库，其中两个来自大型海胆，两个来自小型海胆。共获得88,581个unigenes，平均长度为1354 bp，其中66,331个（74.88%）unigenes可以使用六大公开数据库进行注释。对比分析发现353个unigenes存在差异表达（log2(ratio) >= 1，FDR
Strongylocentrotus nudus is an edible sea urchin, mainly harvested in China. Correlation studies indicated that S. nudus with larger diameter have a prolonged marketing time and better palatability owing to their precocious gonads and extended maturation process. However, the molecular mechanism underlying this phenomenon is still unknown. Here, transcriptome sequencing was applied to study the ovaries of adult S. nudus with different shell diameters to explore the possible mechanism. In this study, four independent cDNA libraries were constructed, including two from the big size urchins and two from the small ones using a HiSeq (TM) 2500 platform. A total of 88,581 unigenes were acquired with a mean length of 1354 bp, of which 66,331 (74.88%) unigenes could be annotated using six major publicly available databases. Comparative analysis revealed that 353 unigenes were differentially expressed (with log2(ratio) >= 1, FDR