MicroRNA-30c functions as a tumor suppressor via targeting SNAI1 in esophageal squamous cell carcinoma
MicroRNA-30c functions as a tumor suppressor via targeting SNAI1 in esophageal squamous cell carcinoma
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MicroRNA-30c 通过靶向 SNAI1 在食管鳞状细胞癌中发挥肿瘤抑制因子的作用
DOI：
10.1016/j.biopha.2017.12.095
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发表时间：
2018-02-01
影响因子：
7.5
通讯作者：
Shao, Yongfeng
中科院分区：
文献类型：
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作者：
Ma, Teng;Zhao, Ye;Shao, Yongfeng
背景：miRNAs的异常表达参与了多种肿瘤的发生、发展和转移。食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）中多种microRNA的研究已取得一定进展，但miR-30 c的研究还未见报道。为了进一步研究其对食管鳞癌细胞的影响，我们完成了一系列实验。检测miR-30 c异位表达对食管鳞癌细胞增殖、迁移和侵袭能力的影响。结果：miR-30 c在食管鳞癌组织和细胞系中表达明显下调，在食管鳞癌中的表达明显下调。临床上，我们发现较低的miR-30 c表达与ESCC进展和生存率显著相关。我们还阐明了miR-30 c抑制ESCC细胞系的细胞增殖、侵袭和上皮向间质转化（EMT）。结论：miR-30 c通过与SNAI 1的3 '-UTR结合，抑制ESCC的生物学行为和EMT进展。因此，miR-30 c可能成为食管鳞癌的一个有前景的生物标志物和治疗靶点。
Background: Aberrant expression of miRNAs was involved in tumor initiation, progression and metastasis in multiple cancers. Many kinds of microRNAs in esophageal squamous cell carcinoma (ESCC) have been researched, whereas miR-30c has not been included.Methods: Firstly, we explored the expression of miR-30c in ESCC tissue and serum samples and its relations to the survival. To further investigate its effects on ESCC cells, we completed a series of experiments. We detected the effects of ectopic miR-30c expression on the proliferation, migration and invasion of ESCC cells in vitro. We identified the target role of SNAI1 in ESCC using Dual-luciferase reporter assay and western blot assay.Results: The results showed miR-30c was significant down-regulated in ESCC tissues and cell lines. Clinically, we found lower miR-30c expression was significantly correlated with worse ESCC progression and survival. Also we clarified that miR-30c suppressed cell proliferation, invasion and epithelial to mesenchymal transition (EMT) of ESCC cell lines. What's more, we figured out that miR-30c inhibits ESCC biological behaviors and EMT progress by directly binding to the 3'-UTR of SNAI1.Conclusion: This study provides new insight into the mechanism responsible for the development of human ESCC. Therefore, miR-30c could be a promising biomarker and a therapeutic target for ESCC in the future.