RinR1B Contains a Ubiquitin-Like Docking Module for Interaction with Cbx Proteins
RinR1B Contains a Ubiquitin-Like Docking Module for Interaction with Cbx Proteins
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DOI：
10.1021/bi901131u
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发表时间：
2009-11-10
期刊：
影响因子：
2.9
通讯作者：
Arrowsmith, Cheryl H.
中科院分区：
文献类型：
--
作者：
Bezsonova, Irina;Walker, John R.;Arrowsmith, Cheryl H.
PcG蛋白是一组特殊的抑制转录因子，参与染色质的表观遗传修饰。它们来自两个不同的功能基团，c，催化活性络合物。多梳抑制复合体I(PRC1)和2(PRO)。Prc1复合体是一个重要的、但尚未得到充分研究的多蛋白质组蛋白泛素化机器，负责通过历史性的H_2A K_(119)修饰维持基因的转录沉默状态。包含PRC1亚单位的环，结构域也有底物靶向，结构域与CBX蛋白相互作用，这些蛋白在染色质和RNA结合中被i-i复制。在收银台工作。我们展示了Ring 113的C-末端结构域的高分辨率结构，揭示了一个具有明显保守表面区的变异的泛素样折叠。油晶体结构的基础和对该结构域的突变分析我们表明，保守的表面负责与形成PRO和同源二聚体的CBX成员的相互作用。这些数据表明，Ring，113作为接头参与了PRC1复合体成员与核小体之间的结合
Polycomb group (PcG) proteins are a special set of repressive transcription Factors involved in epigenetic modifications of chromatin. They from two functionally distinct groups of c,catalytically active complexes. Polycomb repressive complex I (PRC1) and 2 (PRO). The PRC1 complex is an important yet poorly characterized multiprotein histone ubiquitylation machine responsible For maintaining transcriptionally silent states of genes through historic H2A K 119 modification. The Ring, domain containing subunits of PRC1 also have sustrate-targeting, domains that interact with Cbx proteins, which have been i-i-riplicated In chromatin and RNA binding. In till,, work. we present a high resolution structure of the C-terminal domain of Ring 113, revealing a variant ubiquitin-like fold with I distinct conserved surface region. Oil the basis of crystal structure and mutational analysis of this domain we show that the conserved surface is responsible For Interaction with Cbx members of the PRO and homodimer formation These data suggest a mechanism by which Ring, 113 serves as an adaptor that mediates binding between the member of the PRC1 complex and the nucleosome