Coral calcification under environmental change: a direct comparison of the alkalinity anomaly and buoyant weight techniques
Coral calcification under environmental change: a direct comparison of the alkalinity anomaly and buoyant weight techniques
复制标题
环境变化下的珊瑚钙化：碱度异常和浮力技术的直接比较
DOI：
10.1007/s00338-016-1507-z
复制
发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Hoadley, Kenneth D.
中科院分区：
文献类型：
--
作者：
Schoepf, Verena;Hu, Xinping;Holcomb, Michael;Cai, Wei-Jun;Li, Qian;Wang, Yongchen;Xu, Hui;Warner, Mark E.;Melman, Todd F.;Hoadley, Kenneth D.
目前有两种主要方法--浮力重量(BW)和碱度异常(AA)技术--用于量化硬珊瑚的净钙化率(G)。然而，尚不清楚它们是否具有直接可比性，因为迄今为止进行的少数方法比较产生了不一致的结果。此外，还没有对热带珊瑚进行这样的比较。我们直接比较了四种热带和一种温带珊瑚在不同二氧化碳、温度和营养条件下培养的GBWA值和GAA值。评估了进行碱度耗竭培养的一系列方案。对于热带珊瑚，人工搅拌的开顶孵化产生的GAA与GBW高度相关，但没有显著差异。同样，温带珊瑚的GAA与利用泵提供水分运动时的GBW没有显著差异，但当水分运动主要由曝气产生时，GAA显著低于GBW的16%。这表明，这两种技术可以在珊瑚中产生类似的钙化率，但只有在特定条件下进行碱度耗尽孵化时才能产生。对孵化方案提出了一般性建议，特别是关于足够的水运动和孵化时间。此外，对已公布数据的重新分析强调了在两个变量都是随机的且测量有误差的情况下使用适当的回归统计数据的重要性。总体而言，我们推荐AA技术用于群落和短期昼夜钙化的调查，以及BW技术用于测量在较长时间尺度上综合的生物体钙化率，因为这两种方法的实际局限性。我们的发现将有助于直接比较使用这两种方法测量珊瑚钙化的研究，从而对海洋酸化研究和珊瑚生物学领域具有重要意义。
Two primary methods—the buoyant weight (BW) and alkalinity anomaly (AA) techniques—are currently used to quantify net calcification rates (G) in scleractinian corals. However, it remains unclear whether they are directly comparable since the few method comparisons conducted to date have produced inconsistent results. Further, such a comparison has not been made for tropical corals. We directly comparedGBWandGAAin four tropical and one temperate coral species cultured under variouspCO2, temperature, and nutrient conditions. A range of protocols for conducting alkalinity depletion incubations was assessed. For the tropical corals, open-top incubations with manual stirring producedGAAthat were highly correlated with and not significantly different fromGBW. Similarly,GAAof the temperate coral was not significantly different fromGBWwhen incubations provided water motion using a pump, but were significantly lower thanGBWby 16% when water motion was primarily created by aeration. This shows that the two techniques can produce comparable calcification rates in corals but only when alkalinity depletion incubations are conducted under specific conditions. General recommendations for incubation protocols are made, especially regarding adequate water motion and incubation times. Further, the re-analysis of published data highlights the importance of using appropriate regression statistics when both variables are random and measured with error. Overall, we recommend the AA technique for investigations of community and short-term day versus night calcification, and the BW technique to measure organism calcification rates integrated over longer timescales due to practical limitations of both methods. Our findings will facilitate the direct comparison of studies measuring coral calcification using either method and thus have important implications for the fields of ocean acidification research and coral biology in general.
登录
查看更多内容
通过降低环境碳酸根离子浓度抑制石珊瑚骨骼生长：跨科比较
DOI：
--
发表时间：
2003
期刊：
Proceedings of the Royal Society of London. Series B: Biological Sciences
影响因子：
--
作者：
F. Marubini;C. Ferrier‐Pagès;J. Cuif
通讯作者：
J. Cuif
影响因子：
2.7
作者：
Holcomb M;Tambutté E;Allemand D;Tambutté S
通讯作者：
Tambutté S
水流对伯利兹石珊瑚生长和能量的影响
影响因子：
3.5
作者：
K. Sebens;B. Helmuth;E. Carrington;B. Agius
通讯作者：
B. Agius
用于研究珊瑚钙化生理机制的改进 45Ca 协议
影响因子：
2.4
作者：
É. Tambutté;É. Tambutté;D. Allemand;Isabelle Bourge;J. Gattuso;J. Jaubert
通讯作者：
J. Jaubert
DOI：
10.1016/j.jembe.2010.02.007
发表时间：
2010-04-30
影响因子：
2
作者：
Holcomb, Michael;McCorkle, Daniel C.;Cohen, Anne L.
通讯作者：
Cohen, Anne L.