Deoxyribonucleic acid synthesis and its inhibition in mammalian cells cultured from the animal.
Deoxyribonucleic acid synthesis and its inhibition in mammalian cells cultured from the animal.
复制标题
动物培养的哺乳动物细胞中脱氧核糖核酸的合成及其抑制。
DOI：
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发表时间：
1962
影响因子：
4.8
通讯作者：
P. Ove
中科院分区：
文献类型：
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作者：
I. Lieberman;P. Ove
对于多细胞生物体的一些细胞，正常增殖率和潜在增殖率之间存在相对较大的差异。这样的系统似乎对于研究一些体内控制动物细胞增殖速率的机制特别有用。哺乳动物肝脏具有这种特性，可能是这些研究中最常用的工具。培养的哺乳动物细胞由于易于操作且环境相对简单，可能具有整个动物所不具备的优势。然而，即使是在体外增殖很短一段时间的细胞，其价值也值得怀疑，因为它们可能已经发生了重要的变化。此外，使用直接从动物获得的用于培养的细胞具有严重的缺点，即大部分细胞在接种后很快死亡。通过避免细胞的广泛解聚和在培养基中使用血清，从兔肾皮质制备了培养物，其中大约一半的细胞似乎能够合成和增殖脱氧核糖核酸。希望直接从动物培养的肾细胞可能成为研究控制机制的有用工具，因此对一些影响其脱氧核糖核酸合成的因素进行了研究。
With some of the cells of multicellular organisms, a relatively large discrepancy exists between the normal and potential rates of multiplication. Such systems would seem to be particularly useful for studies of some of the mechanisms which operate in viva to control the rates of multiplication of animal cells. Mammalian liver, having this property, has perhaps been the most commonly used tool for these studies. Cultured mammalian cells, because of the ease of their manipulability and the relative simplicity of their environment, might provide advantages not offered by the whole animal. Cells which have multiplied in vitro for even a brief period are, however, of doubtful value because of the possibility that they have already undergone important changes. Further, the use of cells obtained for culture directly from the animal has suffered from the serious disadvantage that a large percentage of the cells die soon after inoculation. By avoiding both extensive disaggregation of cells and the use of serum in the culture medium, cultures have been prepared from rabbit kidney cortex in which approximately one-half the cells appear to be capable of deoxyribonucleic acid synthesis and multiplication. With the hope that kidney cells cultured directly from the animal may prove to be a useful tool for the investigation of control mechanisms, some factors have been studied which influence their synthesis of deoxyribonucleic acid.