An Endophytic Actinomycete, Streptomyces sp. AOK-30, Isolated from Mountain Laurel and Its Antifungal Activity
An Endophytic Actinomycete, Streptomyces sp. AOK-30, Isolated from Mountain Laurel and Its Antifungal Activity
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内生放线菌，链霉菌属 sp。
DOI：
10.1007/pl00013109
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发表时间：
2002
影响因子：
1.2
通讯作者：
H. Kunoh
中科院分区：
文献类型：
--
作者：
T. Nishimura;A. Meguro;Sachiko Hasegawa;Y. Nakagawa;M. Shimizu;H. Kunoh
为探讨山月桂内生放线菌作为山月桂真菌病害生防剂的潜力，对山月桂幼株进行了内生放线菌筛选。被用作隔离源。从供试植物的叶、茎、根中分离到73株放线菌，经鉴定为链霉菌属Streptomycyp.选择AOK-30是因为i）它对杜鹃花科的各种酵母和真菌病原体具有广泛和强烈的抗菌谱，ii）它生长在用于该植物组织培养的增殖和生根培养基上，以及iii）用该分离物处理的组织培养幼苗变得对盘多毛孢病具有抗性，而没有任何不利的生长影响，例如畸形、矮化、变色和落叶。该分离株经鉴定为链霉菌属，基于文化、生理和形态特征。研究结果表明，AOK-30菌株是一种很有潜力的山月桂病害生防菌株。
To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of mountain laurel, young plants of mountain laurel (Kalmia latifoliaL.) were used as an isolation source. From a total of 73 actinomycetes isolates obtained from leaves, stems and roots of test plants,Streptomycessp. AOK-30 was selected, because i) it had a broad and intense antimicrobial spectrum against various yeasts and fungal pathogens of Ericaceae, ii) it grew on the multiplication and rooting media for tissue culture for this plant, and iii) the tissue-cultured seedlings that had been treated with this isolate became resistant to Pestalotia disease without any adverse growth effects such as malformation, dwarfing, discolorization and defoliation. This isolate was identified asStreptomycessp., based on cultural, physiological and morphological characteristics. The present results indicate that isolate AOK-30 is a potential biocontrol agent against diseases of mountain laurel.