CELLULAR AGING OF ALZHEIMERS-DISEASE AND DOWN-SYNDROME CELLS IN CULTURE
CELLULAR AGING OF ALZHEIMERS-DISEASE AND DOWN-SYNDROME CELLS IN CULTURE
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DOI：
10.1016/0921-8734(91)90013-2
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发表时间：
1991-03-01
期刊：
影响因子：
--
通讯作者：
CASSIMAN, JJ
中科院分区：
文献类型：
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作者：
CARMELIET, G;DAVID, G;CASSIMAN, JJ
在阿尔茨海默病中，典型的临床症状和病理结果仅限于神经系统。然而，就像其他一些神经代谢紊乱一样，在周围组织中也发现了几个变化。这项研究的目的是检验可以在体外研究阿尔茨海默病患者皮肤成纤维细胞培养的细胞特性是否与年龄匹配的对照组不同。唐氏综合症患者也包括在内，因为几乎100%的唐氏综合症患者都有相同的神经病理结果。由于阿尔茨海默病是一种与年龄相关的疾病，我们研究了皮肤成纤维细胞培养的生长特征。体外培养的成纤维细胞衰老是一种被广泛接受的体内衰老模型。正常的生长特性。我们可以得出结论，阿尔茨海默病和唐氏综合症都不存在过早衰老，而且在周围组织中发现的异常与疾病本身有关。β-淀粉样前体蛋白(β-APP)已被证明具有粘附性相互作用。因此，我们研究了皮肤成纤维细胞培养中的几个黏附参数：与纤维连接蛋白涂层的黏附，与阿尔茨海默病培养的细胞外基质的黏附，以及黏附相关分子(β-1整合素、细胞表面蛋白多糖、细胞外基质蛋白多糖、细胞外基质纤维连接蛋白)的半定量。在所检测的参数中没有发现显著差异。
In Alzheimer's disease, the typical clinical symptoms and the pathological findings are restricted to the nervous system. Nevertheless, like in some other neurologic-metabolic disorders, several alterations are found in peripheral tissues. The aim of this study was to examine whether cellular properties which can be studied in vitro on skin fibroblast cultures obtained from Alzheimer's disease patients differ from those of age-matched controls. Down syndrome patients were also included, since the same neuropathological findings are present in nearly 100% of Down syndrome patients. Since Alzheimer's disease is an age-related disorder, we examined the growth characteristics of skin fibroblast cultures. The in vitro senescence of cultured fibroblasts is widely accepted as a model for in vivo ageing. Normal growth properties were found. We can conclude that there is no premature ageing in Alzheimer's disease nor in Down syndrome and that the abnormalities found in peripheral tissues are related to the disease itself. The beta-amyloid precursor protein (beta-APP) has been shown to have adhesive interactions. We therefore investigated several parameters of adhesion in the skin fibroblast cultures: adhesion to a fibronectin coat, adhesion to extracellular matrix of Alzheimer's disease cultures and semi-quantification of adhesion-related molecules (beta-1-integrin, cell surface proteoglycans, extracellular matrix proteoglycans, extracellular matrix fibronectin). No significant difference was found in the parameters examined.