Involvement of abscisic acid in ozone-induced puerarin production of Pueraria thomsnii Benth. suspension cell cultures
Involvement of abscisic acid in ozone-induced puerarin production of Pueraria thomsnii Benth. suspension cell cultures
复制标题
脱落酸参与臭氧诱导葛根葛根素的产生。
DOI：
10.1007/s00299-011-1153-4
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发表时间：
2011
影响因子：
6.2
通讯作者：
Maojun Xu
中科院分区：
文献类型：
--
作者：
Lina Sun;Hu Su;Yun Zhu;Maojun Xu
Exposure to ozone induced a rapid increase in the levels of the sesquiterpene phytohormone abscisic acid (ABA) and the isoflavone puerarin in suspension cell cultures ofPueraria thomsniiBenth. The observed increases in ABA and puerarin were dependent on the concentration of ozone applied toP. thomsniicell cultures. In order to examine the role of ABA in ozone-induced puerarin production, cell suspensions were pretreated with the ABA biosynthetic inhibitor fluridone. Following ozone exposure, fluridone treatment suppressed ABA accumulation suggesting ABA was normally synthesized de novo through the carotenoid pathway. Fluridone also blocked ozone-induced puerarin production, which could be reversed through application of exogenous ABA. However, in the absence of ozone, ABA itself had no effect on puerarin accumulation in the suspension cells. Taken together, the data indicate that ozone is an efficient elicitor of puerarin production and may be particularly applicable for improving puerarin production in plant cell cultures. Furthermore, we demonstrate that ABA is one factor associated with ozone-induced puerarin production inP. thomsniicell cultures.
