Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract
Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract
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DOI：
10.1039/d0lc00304b
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发表时间：
2020-05-07
期刊：
影响因子：
6.1
通讯作者：
Cunningham, Brian T.
中科院分区：
文献类型：
--
作者：
Sun, Fu;Ganguli, Anurup;Cunningham, Brian T.
对传染病病原体的快速、灵敏和特异的检测和报告对于患者管理和疫情监测非常重要。我们展示了一个与智能手机集成的护理点系统，用于从鼻拭子介质中检测活病毒，使用一组马呼吸道传染病作为相应人类疾病的模型系统，如新冠肺炎。通过在微流控芯片上进行环介导的等温扩增，扩增了5种病原体的特异性核酸序列，并在反应结束时用智能手机进行检测。使用我们的系统可以正确地诊断病原体添加的马鼻拭子样本，检测限与传统的基于实验室的检测方法聚合酶链式反应相当，结果大约在30分钟内完成。
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in similar to 30 minutes.